Journal ArticleDOI
Size-dependency of nanoparticle-mediated gene transfection: studies with fractionated nanoparticles.
TLDR
The results suggest that the particle size is an important factor, and that the smaller-sized fraction of the nanoparticle formulation predominantly contributes towards their transfection.About:
This article is published in International Journal of Pharmaceutics.The article was published on 2002-09-05. It has received 554 citations till now. The article focuses on the topics: PLGA & Nanoparticle.read more
Citations
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Biodegradable nanoparticles for drug and gene delivery to cells and tissue
Jayanth Panyam,Vinod Labhasetwar +1 more
TL;DR: Based on the above mechanism, various potential applications of nanoparticles for delivery of therapeutic agents to the cells and tissue are discussed.
Journal ArticleDOI
Nonviral Vectors for Gene Delivery
TL;DR: Two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells.
Journal ArticleDOI
Nanoencapsulation I. Methods for preparation of drug-loaded polymeric nanoparticles.
TL;DR: In this review the most important preparation methods are described, especially those that make use of natural polymers, andvantages and disadvantages will be presented so as to facilitate selection of an appropriate nanoencapsulation method according to a particular application.
Journal ArticleDOI
Mechanics of receptor-mediated endocytosis
TL;DR: It is shown that particles in the size range of tens to hundreds of nanometers can enter or exit cells via wrapping even in the absence of clathrin or caveolin coats, and an optimal particles size exists for the smallest wrapping time.
Journal ArticleDOI
Nanoparticles for drug delivery: the need for precision in reporting particle size parameters.
TL;DR: The present review highlights the difficulties in validating the data from biodistribution studies without accurate particle size determination.
References
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Journal ArticleDOI
Polymeric system for dual growth factor delivery
TL;DR: This is the first report of a vehicle capable of delivery of multiple angiogenic factors with distinct kinetics, and these results clearly indicate the importance of multiple growth factor action in tissue regeneration and engineering.
Journal ArticleDOI
Chitosan-DNA nanoparticles as gene carriers : synthesis, characterization and transfection efficiency
Hai-Quan Mao,Krishnendu Roy,Vu L. Troung-Le,Kevin A. Janes,Kevin Y. Lin,Yan Wang,J. Thomas August,Kam W. Leong +7 more
TL;DR: The chitosan-DNA nanoparticles could partially protect the encapsulated plasmid DNA from nuclease degradation as shown by electrophoretic mobility analysis and three different schemes to conjugate transferrin or KNOB protein to the nanoparticle surface were developed.
Journal ArticleDOI
Oral gene delivery with chitosan--DNA nanoparticles generates immunologic protection in a murine model of peanut allergy.
TL;DR: It is demonstrated that oral allergen-gene immunization with chitosan–DNA nanoparticles is effective in modulating murine anaphylactic responses, and its prophylactic utility in treating food allergy is indicated.
Journal ArticleDOI
Rapid endo-lysosomal escape of poly(dl-lactide-co-glycolide) nanoparticles: implications for drug and gene delivery
TL;DR: The mechanism of rapid escape is by selective reversal of the surface charge of NPs (from anionic to cationic) in the acidic endolysosomal compartment, which causes the NPs to interact with the endo‐lysosomal membrane and escape into the cytosol.
Journal ArticleDOI
Residual polyvinyl alcohol associated with poly (D,L-lactide-co-glycolide) nanoparticles affects their physical properties and cellular uptake.
TL;DR: The residual PVA associated with nanoparticles is an important formulation parameter that can be used to modulate the pharmaceutical properties of PLGA nanoparticles.
Related Papers (5)
Biodegradable nanoparticles for drug and gene delivery to cells and tissue
Jayanth Panyam,Vinod Labhasetwar +1 more