Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq
James W. Wilson,C. M. Ott,K. Höner zu Bentrup,Rajee Ramamurthy,L. Quick,Steffen Porwollik,Pui Cheng,Michael McClelland,George Tsaprailis,Timothy R. Radabaugh,Andrea M. Hunt,D. Fernandez,Emily G. Richter,Miti Shah,Michelle Kilcoyne,Lokesh Joshi,Mayra Nelman-Gonzalez,S. Hing,Macarena Parra,P. Dumars,Kelly L.L. Norwood,R. Bober,J. Devich,A. Ruggles,Carla Goulart,Mark Rupert,Louis S. Stodieck,Phillip Stafford,L. Catella,Michael J. Schurr,Michael J. Schurr,Kent L. Buchanan,Kent L. Buchanan,Lisa A. Morici,James McCracken,James McCracken,Patricia L. Allen,Patricia L. Allen,C. Baker-Coleman,C. Baker-Coleman,Timothy G. Hammond,Timothy G. Hammond,Jörg Vogel,Randall W. Nelson,Duane L. Pierson,Heidemarie M. Stefanyshyn-Piper,C. A. Nickerson +46 more
TLDR
Strategies to target Hfq and related regulators could potentially decrease infectious disease risks during space flight missions and provide novel therapeutic options on Earth.Abstract:
A comprehensive analysis of both the molecular genetic and phenotypic responses of any organism to the space flight environment has never been accomplished because of significant technological and logistical hurdles. Moreover, the effects of space flight on microbial pathogenicity and associated infectious disease risks have not been studied. The bacterial pathogen Salmonella typhimurium was grown aboard Space Shuttle mission STS-115 and compared with identical ground control cultures. Global microarray and proteomic analyses revealed that 167 transcripts and 73 proteins changed expression with the conserved RNA-binding protein Hfq identified as a likely global regulator involved in the response to this environment. Hfq involvement was confirmed with a ground-based microgravity culture model. Space flight samples exhibited enhanced virulence in a murine infection model and extracellular matrix accumulation consistent with a biofilm. Strategies to target Hfq and related regulators could potentially decrease infectious disease risks during space flight missions and provide novel therapeutic options on Earth.read more
Citations
More filters
Book
Conflict of Interest in Medical Research, Education, and Practice
Bernard Lo,Marilyn J. Field +1 more
TL;DR: Conflict of Interest in Medical Research, Education, and Practice makes several recommendations for strengthening conflict of interest policies and curbing relationships that create risks with little benefit.
Journal ArticleDOI
Deep sequencing analysis of small noncoding RNA and mRNA targets of the global post-transcriptional regulator, Hfq.
Alexandra Sittka,Sacha Lucchini,Kai Papenfort,Cynthia M. Sharma,Katarzyna Rolle,Tim T. Binnewies,Jay C. D. Hinton,Jörg Vogel +7 more
TL;DR: This work found that the mRNAs of hilD (master regulator of the SPI-1 invasion genes) and flhDC (flagellar master regulator) were bound by Hfq, and presented a generic strategy that is ideal for the systems-level analysis of the post-transcriptional regulons of RNA-binding proteins and for sRNA discovery in a wide range of bacteria.
Journal ArticleDOI
The role of Hfq in bacterial pathogens
Yanjie Chao,Jörg Vogel +1 more
TL;DR: RNomics and deep sequencing approaches discovered the small RNA and mRNA targets of Hfq, and indicated that this protein might impact on the expression of up to 20% of all genes in some organisms, including genes of type 3 secretion systems.
Journal ArticleDOI
Cubesats: Cost-effective science and technology platforms for emerging and developing nations
TL;DR: The development, operation, and analysis of data from cubesats can promote science education and spur technology utilization in emerging and developing nations as mentioned in this paper, which has led more than 80 universities and several emerging nations to develop programs in this field.
Journal ArticleDOI
Acclimation during space flight: Effects on human physiology
TL;DR: See related review by Thirsk and colleagues, page [1324][1]
References
More filters
Journal ArticleDOI
An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database.
TL;DR: The approach described in this manuscript provides a convenient method to interpret tandem mass spectra with known sequences in a protein database.
Journal ArticleDOI
TANDEM: matching proteins with tandem mass spectra
Robertson Craig,Ronald C. Beavis +1 more
TL;DR: The application TANDEM was written to provide the proteomics research community with a set of components that can be used to test new methods and algorithms for performing this type of sequence-to-data matching.
Journal ArticleDOI
A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli.
Eric Massé,Susan Gottesman +1 more
TL;DR: RyhB provides a mechanism for the cell to down-regulate iron-storage proteins and nonessential ironcontaining proteins when iron is limiting, thus modulating intracellular iron usage to supplement mechanisms for iron uptake directly regulated by Fur.
Journal ArticleDOI
Selective silencing of foreign DNA with low GC content by the H-NS protein in Salmonella.
William Wiley Navarre,Steffen Porwollik,Yipeng Wang,Michael McClelland,Henry Rosen,Stephen J. Libby,Ferric C. Fang +6 more
TL;DR: H-NS provides a previously unrecognized mechanism of bacterial defense against foreign DNA, enabling the acquisition of DNA from exogenous sources while avoiding detrimental consequences from unregulated expression of newly acquired genes.
Related Papers (5)
Media Ion Composition Controls Regulatory and Virulence Response of Salmonella in Spaceflight
James W. Wilson,C. Mark Ott,Laura N. Quick,Laura N. Quick,Richard R. Davis,Kerstin Höner zu Bentrup,Aurélie Crabbé,Emily G. Richter,Shameema Sarker,Jennifer Barrila,Steffen Porwollik,Pui Cheng,Michael McClelland,George Tsaprailis,Timothy R. Radabaugh,Andrea M. Hunt,Miti Shah,Mayra Nelman-Gonzalez,Steve Hing,Macarena Parra,Paula Dumars,Kelly L.L. Norwood,Ramona Bober,Jennifer Devich,Ashleigh Ruggles,Autumn CdeBaca,Satro Narayan,Joseph Benjamin,Carla Goulart,Mark Rupert,Luke Catella,Michael J. Schurr,Kent L. Buchanan,Lisa A. Morici,James McCracken,Marc D. Porter,Duane L. Pierson,Scott M. Smith,Max Mergeay,Natalie Leys,Heidemarie M. Stefanyshyn-Piper,Dominic Gorie,Cheryl A. Nickerson +42 more