© 1989
Nature
Publishing
Group
© 1989
Nature
Publishing
Group
© 1989
Nature
Publishing
Group
© 1989
Nature
Publishing
Group
© 1989
Nature
Publishing
Group
Structural parts involved in activation and inactivation of the sodium channel.
Walter Stühmer,Franco Conti,Harukazu Suzuki,Xiaodong Wang,Masaharu Noda,Naoki Yahagi,Hideo Kubo,Shosaku Numa +7 more
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TLDR
Evidence is provided that the positive charges in segment S4 are involved in the voltage–sensing mechanism for activation of the channel and that the region between repeats III and IV is important for its inactivation.Abstract:
Structure-function relationships of the sodium channel expressed in Xenopus oocytes have been investigated by the combined use of site-directed mutagenesis and patch-clamp recording. This study provides evidence that the positive charges in segment S4 are involved in the voltage-sensing mechanism for activation of the channel and that the region between repeats III and IV is important for its inactivation.read more
Citations
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Book ChapterDOI
Ion Channels in Excitable Membranes
TL;DR: The action potential is triggered when the membrane potential, which was at the resting level, depolarizes and reaches the threshold of excitation, which triggers the action potential.
Journal ArticleDOI
From Ionic Currents to Molecular Mechanisms: The Structure and Function of Voltage-Gated Sodium Channels
TL;DR: Together, these studies showed that the mechanisms of sodium channel function and regulation, purified sodium channel protein contained the essential and gives a perspective for future research on the ex-elements for ion conduction and voltage-dependent panding family of Sodium channel proteins.
Journal ArticleDOI
Genetic basis and molecular mechanism for idiopathic ventricular fibrillation
Qiuyun Chen,Glenn E. Kirsch,Danmei Zhang,Ramon Brugada,Josep Brugada,Pedro Brugada,Domenico Potenza,Angel Moya,Martin Borggrefe,Günter Breithardt,Rocio Ortiz-Lopez,Zhiqing Wang,Charles Antzelevitch,Richard E. O'Brien,Eric Schulze-Bahr,Mark T. Keating,Jeffrey A. Towbin,Qing Wang +17 more
TL;DR: It is shown that sodium channels with the missense mutation recover from inactivation more rapidly than normal and that the frameshift mutation causes the sodium channel to be non-functional.
Journal ArticleDOI
SCN5A mutations associated with an inherited cardiac arrhythmia, long QT syndrome
Qing Kenneth Wang,Jiaxiang Shen,Jiaxiang Shen,Igor Splawski,Donald L. Atkinson,Donald L. Atkinson,Zhizhong Li,Jennifer L. Robinson,Arthur J. Moss,Jeffrey A. Towbin,Mark T. Keating +10 more
TL;DR: Genetic linkage between LQT3 and polymorphisms within SCN5A, the cardiac sodium channel gene, and single strand conformation polymorphism and DNA sequence analyses suggest that mutations in SCN 5A cause chromosome 3-linked LQt and indicate a likely cellular mechanism for this disorder.
Journal ArticleDOI
Biophysical and molecular mechanisms of shaker potassium channel inactivation
TL;DR: A region near the amino terminus with an important role in inactivation has been identified and the results suggest a model where this region forms a cytoplasmic domain that interacts with the open channel to cause inactivation.
References
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Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
A quantitative description of membrane current and its application to conduction and excitation in nerve
A. L. Hodgkin,A. F. Huxley +1 more
TL;DR: This article concludes a series of papers concerned with the flow of electric current through the surface membrane of a giant nerve fibre by putting them into mathematical form and showing that they will account for conduction and excitation in quantitative terms.
Book
Ionic channels of excitable membranes
TL;DR: The Ionic Channel of Excitable Membranes (ICOMB) as discussed by the authors is an extended version of ICOMB with new chapters on fast chemical synapses, modulation through G protein coupled receptors and second messenger systems, molecules cloning, site directed mutagenesis, and cell biology.
BookDOI
Single-channel recording
Bert Sakmann,Erwin Neher +1 more
TL;DR: A Practical Guide to Patch Clamping R.H. Penner, C.W. Heinemann, and P. Jonas.
Journal ArticleDOI
Primary structure of the receptor for calcium channel blockers from skeletal muscle
Tsutomu Tanabe,Hiroshi Takeshima,Atsushi Mikami,Veit Flockerzi,Hideo Takahashi,Kenji Kangawa,Masayasu Kojima,Hisayuki Matsuo,Tadaaki Hirose,Shosaku Numa +9 more
TL;DR: Structural and sequence similarities to the voltage-dependent sodium channel suggest that in the transverse tubule membrane of skeletal muscle the dihydropyridine receptor may act both as voltage sensor in excitation-contraction coupling and as a calcium channel.
Related Papers (5)
A quantitative description of membrane current and its application to conduction and excitation in nerve
A. L. Hodgkin,A. F. Huxley +1 more