Journal ArticleDOI
Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence.
Masaharu Noda,Shin Shimizu,Tsutomu Tanabe,Toshiyuki Takai,Toshiaki Kayano,Takayuki Ikeda,Hideo Takahashi,Hitoshi Nakayama,Yuichi Kanaoka,Naoto Minamino,Kenji Kangawa,Hisayuki Matsuo,Michael A. Raftery,Tadaaki Hirose,Seiichi Inayama,Hidenori Hayashida,Takashi Miyata,Shosaku Numa +17 more
TLDR
Cloning and sequence analysis of cDNA for the Electrophorus electricus electroplax sodium channel indicate that this protein exhibits four repeated homology units, which are presumably oriented in a pseudosymmetric fashion across the membrane.Abstract:
Cloning and sequence analysis of cDNA for the Electrophorus electricus electroplax sodium channel indicate that this protein, consisting of 1,820 amino acid residues, exhibits four repeated homology units, which are presumably oriented in a pseudosymmetric fashion across the membrane. Each homology unit contains a unique segment with clustered positively charged residues, which may be involved in the gating structure, possibly in conjunction with negatively charged residues clustered elsewhere.read more
Citations
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Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA.
John R. Riordan,Johanna M. Rommens,Batsheva Kerem,Noa Alon,Richard Rozmahel,Zbyszko Grzelczak,Julian Zielenski,Si Lok,Natasa Plavsic,Jia Ling Chou,Mitchell L. Drumm,Michael C. Iannuzzi,Francis S. Collins,Lap-Chee Tsui +13 more
TL;DR: A deletion of three base pairs that results in the omission of a phenylalanine residue at the center of the first predicted nucleotide-binding domain was detected in CF patients.
Journal ArticleDOI
From Ionic Currents to Molecular Mechanisms: The Structure and Function of Voltage-Gated Sodium Channels
TL;DR: Together, these studies showed that the mechanisms of sodium channel function and regulation, purified sodium channel protein contained the essential and gives a perspective for future research on the ex-elements for ion conduction and voltage-dependent panding family of Sodium channel proteins.
Journal ArticleDOI
X-ray structure of a voltage-dependent K+ channel
Youxing Jiang,Alice Lee,Jiayun Chen,Vanessa Ruta,Martine Cadene,Brian T. Chait,Roderick MacKinnon +6 more
TL;DR: The structure of KvAP, a voltage-dependent K+ channel from Aeropyrum pernix, is presented and a crystal structure of the full-length channel at a resolution of 3.2 Å is determined, which suggests that the voltage-sensor paddles move in response to membrane voltage changes, carrying their positive charge across the membrane.
Journal ArticleDOI
The physiology of excitatory amino acids in the vertebrate central nervous system.
Mark L. Mayer,Gary L. Westbrook +1 more
TL;DR: In this article, the authors propose a method to solve the problem of unstructured data.Abbreviations 198 and 198.3.1.5.1].
Journal ArticleDOI
Sequence and structure of a human glucose transporter
Mike Mueckler,C Caruso,Stephen A. Baldwin,Maria Panico,I Blench,Howard R. Morris,WJ Allard,Gustav E. Lienhard,Harvey F. Lodish +8 more
TL;DR: Structural analysis of the purified human erythrocyte glucose transporter by fast atom bombardment mapping and gas phase Edman degradation confirmed the identity of the clone and demonstrated that the HepG2 and ery Throcyte transporters are highly homologous and may be identical.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
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A simple method for displaying the hydropathic character of a protein
Jack Kyte,Russell F. Doolittle +1 more
TL;DR: A computer program that progressively evaluates the hydrophilicity and hydrophobicity of a protein along its amino acid sequence has been devised and its simplicity and its graphic nature make it a very useful tool for the evaluation of protein structures.
Journal ArticleDOI
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
TL;DR: In this article, the rat pancreas RNA was used as a source for the purification of alpha-amylase messenger ribonucleic acid (RBA) using 2-mercaptoethanol.
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