Subepithelial telocytes are an important source of Wnts that supports intestinal crypts
Michal Shoshkes-Carmel,Yue J. Wang,Kirk J. Wangensteen,Beáta Tóth,Ayano Kondo,Efi E. Massasa,Shalev Itzkovitz,Klaus H. Kaestner +7 more
TLDR
Subepithelial telocytes are identified as a source of Wnt signals that enable proliferation and differentiation of intestinal stem cells, an essential function for maintenance of the intestinal epithelium.Abstract:
Tissues that undergo rapid cellular turnover, such as the mammalian haematopoietic system or the intestinal epithelium, are dependent on stem and progenitor cells that proliferate to provide differentiated cells to maintain organismal health. Stem and progenitor cells, in turn, are thought to rely on signals and growth factors provided by local niche cells to support their function and self-renewal. Several cell types have been hypothesized to provide the signals required for the proliferation and differentiation of the intestinal stem cells in intestinal crypts1-6. Here we identify subepithelial telocytes as an important source of Wnt proteins, without which intestinal stem cells cannot proliferate and support epithelial renewal. Telocytes are large but rare mesenchymal cells that are marked by expression of FOXL1 and form a subepithelial plexus that extends from the stomach to the colon. While supporting the entire epithelium, FOXL1+ telocytes compartmentalize the production of Wnt ligands and inhibitors to enable localized pathway activation. Conditional genetic ablation of porcupine (Porcn), which is required for functional maturation of all Wnt proteins, in mouse FOXL1+ telocytes causes rapid cessation of Wnt signalling to intestinal crypts, followed by loss of proliferation of stem and transit amplifying cells and impaired epithelial renewal. Thus, FOXL1+ telocytes are an important source of niche signals to intestinal stem cells.read more
Citations
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Thyroid Hormone-Activated Signaling Pathways are Essential for Development of Intestinal Stem Cells.
TL;DR: Using thyroid hormone (TH) -dependent intestinal remodeling during amphibian metamorphosis as a model to study these mechanisms, this paper found that several signaling pathways, including the SHH/BMP4, WNT, Notch, and Hippo pathways, are regulated by TH and involved in stem cell regulation.
Journal ArticleDOI
Synthesis and Physiological Remodeling of CD34 Cells in the Skin following the Reversal of Fibrosis through Intensive Treatment for Lower Limb Lymphedema: A Case Report
José Maria Pereira de Godoy,Ana Carolina Pereira de Godoy,Maria de Fatima Guerreiro Godoy,Dalisio de Santi Neto +3 more
TL;DR: In this article , a clinical trial involving the evaluation of changes in immunostained CD34 cells in the epidermis and dermis of a patient before and after intensive treatment for clinical stage II lymphedema was conducted using the Godoy Method, which was adapted to the treatment of skin fibrosis.
Journal ArticleDOI
Morphologies and potential roles of telocytes in nervous tissue
TL;DR: A mini-review of telocytes in diverse organs and tissues, focusing on their potential functions in nervous tissue and current challenges in investigating TCs as a distinct cell type is presented in this article .
Posted ContentDOI
Telocytes are a critical source of Wnts essential for hair follicle regeneration
TL;DR: In this paper , the authors show that telocytes, unique interstitial cells that harbor long cytoplasmic extensions, are obligatory Wnt-producing niche cells in the hair follicle, and they reveal a continuous network of interconnected telocytes that envelopes stem cells and their progeny along the path of differentiation.
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Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts
Toshiro Sato,Johan H. van Es,Hugo J. Snippert,Daniel E. Stange,Robert G.J. Vries,Maaike van den Born,Nick Barker,Noah F. Shroyer,Marc van de Wetering,Hans Clevers +9 more
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