The HMI™ module: a new tool to study the Host-Microbiota Interaction in the human gastrointestinal tract in vitro
Massimo Marzorati,Barbara Vanhoecke,Tine De Ryck,Mehdi Sadaghian Sadabad,Iris Pinheiro,Sam Possemiers,Pieter Van den Abbeele,Lara Derycke,Marc Bracke,Jan Pieters,Tom Hennebel,Hermie J. M. Harmsen,Willy Verstraete,Tom Van de Wiele +13 more
TLDR
The HMI module offers the possibility of co-culturing a gut representative microbial community with enterocyte-like cells up to 48 h and may therefore contribute to the mechanistic understanding of host-microbiome interactions.Abstract:
Background: Recent scientific developments have shed more light on the importance of the host-microbe interaction, particularly in the gut. However, the mechanistic study of the host-microbe interplay is complicated by the intrinsic limitations in reaching the different areas of the gastrointestinal tract (GIT) in vivo. In this paper, we present the technical validation of a new device - the Host-Microbiota Interaction (HMI) module - and the evidence that it can be used in combination with a gut dynamic simulator to evaluate the effect of a specific treatment at the level of the luminal microbial community and of the host surface colonization and signaling. Results: The HMI module recreates conditions that are physiologically relevant for the GIT: i) a mucosal area to which bacteria can adhere under relevant shear stress (3 dynes cm �2 ); ii) the bilateral transport of low molecular weight metabolites (4 to 150 kDa) with permeation coefficients ranging from 2.4 × 10 �6 to 7.1 × 10 �9 cm sec �1 ; and iii) microaerophilic conditions at the bottom of the growing biofilm (PmO2 =2.5 ×1 0 �4 cm sec �1 ). In a long-term study, the host’s cells in the HMI module were still viable after a 48-hour exposure to a complex microbial community. The dominant mucus-associated microbiota differed from the luminal one and its composition was influenced by the treatment with a dried product derived from yeast fermentation. The latter - with known anti-inflammatory properties induced a decrease of pro-inflammatory IL-8 production between 24 and 48 h. Conclusions: The study of the in vivo functionality of adhering bacterial communities in the human GIT and of the localized effect on the host is frequently hindered by the complexity of reaching particular areas of the GIT. The HMI module offers the possibility of co-culturing a gut representative microbial community with enterocyte-like cells up to 48 h and may therefore contribute to the mechanistic understanding of host-microbiome interactions.read more
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References
More filters
Journal ArticleDOI
Diversity of the human intestinal microbial flora.
Paul B. Eckburg,Elisabeth M. Bik,Charles N. Bernstein,Elizabeth Purdom,Les Dethlefsen,Michael Sargent,Steven R. Gill,Karen E. Nelson,David A. Relman,David A. Relman,David A. Relman +10 more
TL;DR: A majority of the bacterial sequences corresponded to uncultivated species and novel microorganisms, and significant intersubject variability and differences between stool and mucosa community composition were discovered.
Journal ArticleDOI
The gut flora as a forgotten organ
Ann M O'Hara,Fergus Shanahan +1 more
TL;DR: The flora has a collective metabolic activity equal to a virtual organ within an organ, and the mechanisms underlying the conditioning influence of the bacteria on mucosal homeostasis and immune responses are beginning to be unravelled.
Journal ArticleDOI
Diversity, metabolism and microbial ecology of butyrate-producing bacteria from the human large intestine.
Petra Louis,Harry J. Flint +1 more
TL;DR: This review provides an overview of the current knowledge of the diversity, metabolism and microbial ecology of this functionally important group of bacteria and suggests that mechanisms proposed recently in non-gut Clostridium spp.
Journal ArticleDOI
Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow
Hyun Jung Kim,Dongeun Huh,Geraldine A. Hamilton,Donald E. Ingber,Donald E. Ingber,Donald E. Ingber +5 more
TL;DR: This gut-on-a-chip recapitulates multiple dynamic physical and functional features of human intestine that are critical for its function within a controlled microfluidic environment that is amenable for transport, absorption, and toxicity studies, and hence it should have great value for drug testing as well as development of novel intestinal disease models.
Journal ArticleDOI
Genes and Molecules of Lactobacilli Supporting Probiotic Action
TL;DR: This review addresses the advances in the understanding of the probiotic-host interaction with a focus on the molecular microbiology of lactobacilli, facing the challenge of molecular characterization of probiotic traits.
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