The lin-4 Regulatory RNA Controls Developmental Timing in Caenorhabditis elegans by Blocking LIN-14 Protein Synthesis after the Initiation of Translation
Philip H. Olsen,Victor R. Ambros +1 more
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TLDR
In this article, the authors investigated the mechanism of lin-4 RNA action by examining the fate of Lin-14 mRNA in vivo during the time that lin4 RNA is expressed, and they found that association of lin 4 RNA with the 3* untranslated region (UTR) of Lin 14 mRNA permits normal biogenesis of lin 14 mRNA, and normal translational initiation, but inhibits step(s) thereafter, such as translational elongation and/or release of stable LIN-14 protein.About:
This article is published in Developmental Biology.The article was published on 1999-12-15 and is currently open access. It has received 1134 citations till now. The article focuses on the topics: Small RNA & Translational regulation.read more
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MicroRNAs: Genomics, Biogenesis, Mechanism, and Function
TL;DR: Although they escaped notice until relatively recently, miRNAs comprise one of the more abundant classes of gene regulatory molecules in multicellular organisms and likely influence the output of many protein-coding genes.
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The functions of animal microRNAs
TL;DR: Evidence is mounting that animal miRNAs are more numerous, and their regulatory impact more pervasive, than was previously suspected.
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MicroRNAs: small RNAs with a big role in gene regulation
Lin He,Gregory J. Hannon +1 more
TL;DR: Two founding members of the microRNA family were originally identified in Caenorhabditis elegans as genes that were required for the timed regulation of developmental events and indicate the existence of multiple RISCs that carry out related but specific biological functions.
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Oncomirs — microRNAs with a role in cancer
TL;DR: Evidence has shown that miRNA mutations or mis-expression correlate with various human cancers and indicates that miRNAs can function as tumour suppressors and oncogenes.
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Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight?
TL;DR: This Review summarizes the current understanding of the mechanistic aspects of microRNA-induced repression of translation and discusses some of the controversies regarding different modes of micro RNA function.
References
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The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
TL;DR: Two small lin-4 transcripts of approximately 22 and 61 nt were identified in C. elegans and found to contain sequences complementary to a repeated sequence element in the 3' untranslated region (UTR) of lin-14 mRNA, suggesting that lin- 4 regulates lin- 14 translation via an antisense RNA-RNA interaction.
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A simple, rapid, and sensitive DNA assay procedure.
C Labarca,Kenneth Paigen +1 more
TL;DR: The dissociation of chromatin is critical to accurate determinations of DNA in biological materials using this method, which can detect as little as 10 ng of DNA with rather unsophisticated instrumentation.
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Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans
TL;DR: It is demonstrated that a temporal gradient in Lin-14 protein is generated posttranscriptionally by multiple elements in the lin-14 3'UTR that are regulated by the heterochronic gene Lin-4.
Book
The Nematode Caenorhabditis elegans
TL;DR: This "Book of the Worm" serves as a reference source for C. elegans investigators as well as an introductory monograph for other biologists.
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The Cold Shock Domain Protein LIN-28 Controls Developmental Timing in C. elegans and Is Regulated by the lin-4 RNA
TL;DR: In this article, Lin-28 encodes a cytoplasmic protein with a cold shock domain and retroviral-type (CCHC) zinc finger motifs, consistent with a role for LIN-28 in posttranscriptional regulation.