Journal ArticleDOI
The transcription factor TTF-1 is expressed at the onset of thyroid and lung morphogenesis and in restricted regions of the foetal brain.
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TLDR
The early detection of TTF-1 in the endodermal cells of the thyroid and lung anlage and in restricted neuroblast populations indicates that T TF-1 may have a role in cell determination in these three systems and that additional mechanisms may be involved in the activation of thyroid-specific gene expression.Abstract:
TTF-1, a homeodomain-containing transcription factor, which is required for the specific expression of the thyroglobulin and thyroperoxidase gene promoters in differentiated thyroid cell lines, is expressed at the very beginning of rat thyroid differentiation. TTF-1 mRNA is detected in the endodermal cells of the thyroid rudiment in the rat embryo and precedes the expression of the two known target genes by 5 days. No delay is observed between the appearance of TTF-1 mRNA and protein, which shows a clear nuclear localization. In the adult thyroid, TTF-1 is present only in the endoderm-derived follicular cells. Two additional domains of expression of TTF-1 have been observed, the lung and restricted areas of the brain. In the lung, TTF-1 mRNA and protein are also present at the earliest stages of differentiation and are later confined to the bronchial epithelium. In the brain, TTF-1 appears to be restricted to structures of diencephalic origin, including the developing neurohypophysis. The early detection of TTF-1 in the endodermal cells of the thyroid and lung anlage and in restricted neuroblast populations indicates that TTF-1 may have a role in cell determination in these three systems and that additional mechanisms may be involved in the activation of thyroid-specific gene expression.read more
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Journal ArticleDOI
The T/ebp null mouse: thyroid-specific enhancer-binding protein is essential for the organogenesis of the thyroid, lung, ventral forebrain, and pituitary.
Shioko Kimura,Yoshinobu Hara,Thierry Pineau,Pedro M. Fernández-Salguero,Cecil H. Fox,Jerrold M. Ward,Frank J. Gonzalez +6 more
TL;DR: In situ hybridization showed that the T/ebp gene is expressed in the normal thyroid, lung bronchial epithelium, and specific areas of the forebrain during early embryogenesis, establishing that the expression of T/EBP, a transcription factor known to control thyroid-specific gene transcription, is also essential for organogenesis of the thyroid, lungs, ventral forebrain, and pituitary.
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Nkx-2.5: a novel murine homeobox gene expressed in early heart progenitor cells and their myogenic descendants.
TL;DR: The expression data implicate Nkx-2.5 in commitment to and/or differentiation of the myocardial lineage and demonstrate that cardiogenic progenitors can be distinguished at a molecular level by late gastrulation.
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Pallial and subpallial derivatives in the embryonic chick and mouse telencephalon, traced by the expression of the genes Dlx-2, Emx-1, Nkx-2.1, Pax-6, and Tbr-1
Luis Puelles,Ellen Kuwana,Eduardo Puelles,Alessandro Bulfone,Kenji Shimamura,Jerry Keleher,Susan Smiga,John L.R. Rubenstein +7 more
TL;DR: Differences in the expression topography of Tbr‐1 and Emx‐1 suggest the existence of a novel “ventral pallium” subdivision, which is an EmX‐1‐negative pallial territory intercalated between the striatum and the lateral pallium.
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Development of definitive endoderm from embryonic stem cells in culture
Atsushi Kubo,Katsunori Shinozaki,John M. Shannon,Valerie Kouskoff,Marion Kennedy,Savio L. C. Woo,Hans Joerg Fehling,Gordon Keller +7 more
TL;DR: It is demonstrated that endoderm develops from a brachyury+ population that also displays mesoderm potential, and this differentiation system is established as a unique murine model for studying the development and specification of this germ layer.
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Directed differentiation of telencephalic precursors from embryonic stem cells
Kiichi Watanabe,Daisuke Kamiya,Ayaka Nishiyama,Tomoko Katayama,Satoshi Nozaki,Hiroshi Kawasaki,Yasuyoshi Watanabe,Kenji Mizuseki,Yoshiki Sasai +8 more
TL;DR: In the absence of caudalizing signals, floating aggregates of ES cells generate naive telencephalic precursors that acquire subregional identities by responding to extracellular patterning signals.
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Journal ArticleDOI
A tissue-specific transcription factor containing a homeodomain specifies a pituitary phenotype.
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The pituitary-specific transcription factor GHF-1 is a homeobox-containing protein
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Journal ArticleDOI
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Journal ArticleDOI
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