Three-Dimensional Cell Cultures in Drug Discovery and Development.
Ye Fang,Richard M. Eglen +1 more
- Vol. 22, Iss: 5, pp 456-472
TLDR
This review of leading 3D cell culture technologies and their impact on drug discovery, including spheroids, organoids, scaffolds, hydrogels, organs-on-chips, and 3D bioprinting, discusses the implementation of these technologies in compound identification, screening, and development.Abstract:
The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures as systems that better mimic in vivo physiology. Today, 3D cell cultures are emerging, not only as a new tool in early drug discovery but also as potential therapeutics to treat disease. In this review, we assess leading 3D cell culture technologies and their impact on drug discovery, including spheroids, organoids, scaffolds, hydrogels, organs-on-chips, and 3D bioprinting. We also discuss the implementation of these technologies in compound identification, screening, and development, ranging from disease modeling to assessment of efficacy and safety profiles.read more
Citations
More filters
Organ Printing: Tissue Spheroids as Building Blocks
Vladimir Mironov,Jing Zhang,Carmine Gentile,K Brakke,Thomas C. Trusk,Karoly Jakab,Gabor Forgacs,Vladimirs Kasjanovs,Richard P. Visconti,Roger R. Markwald +9 more
TL;DR: Organ printing can be defined as layer-by-layer additive robotic biofabrication of three-dimensional functional living macrotissues and organ constructs using tissue spheroids as building blocks.
Journal ArticleDOI
Halfway between 2D and Animal Models: Are 3D Cultures the Ideal Tool to Study Cancer-Microenvironment Interactions?
TL;DR: A comprehensive review of the different 3D methods developed recently is presented, and the pros and cons of 3D culture compared to 2D when studying interactions between cancer cells and their microenvironment are discussed.
Journal ArticleDOI
3D bioprinting of functional tissue models for personalized drug screening and in vitro disease modeling
TL;DR: This review presents state-of-the-art 3D bioprinting techniques and discusses the choice of cell source and biomaterials for building functional tissue models that can be used for personalized drug screening and disease modeling.
Dissertation
Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5⁺ stem cell
Journal ArticleDOI
Three-dimensional cell culture systems as an in vitro platform for cancer and stem cell modeling.
TL;DR: This review provides a comprehensive compendium of recent advancements in culturing cells, in particular cancer and stem cells, using 3D culture techniques, and the major approaches highlighted here include cell spheroids, hydrogel embedding, bioreactors, scaffolds, and bioprinting.
References
More filters
Journal ArticleDOI
Hallmarks of cancer: the next generation.
TL;DR: Recognition of the widespread applicability of these concepts will increasingly affect the development of new means to treat human cancer.
Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
Journal ArticleDOI
The origins and the future of microfluidics
TL;DR: The manipulation of fluids in channels with dimensions of tens of micrometres — microfluidics — has emerged as a distinct new field that has the potential to influence subject areas from chemical synthesis and biological analysis to optics and information technology.
Journal ArticleDOI
3D bioprinting of tissues and organs
Sean V. Murphy,Anthony Atala +1 more
TL;DR: 3D bioprinting is being applied to regenerative medicine to address the need for tissues and organs suitable for transplantation and developing high-throughput 3D-bioprinted tissue models for research, drug discovery and toxicology.
Journal ArticleDOI
Can the pharmaceutical industry reduce attrition rates
Ismail Kola,John Landis +1 more
TL;DR: The pharmaceutical industry faces considerable challenges, both politically and fiscally, and the fiscal pressures that face the industry from the perspective of R&D are dealt with.