Transcriptome Profiling, Molecular Biological, and Physiological Studies Reveal a Major Role for Ethylene in Cotton Fiber Cell Elongation
Yong-Hui Shi,Shengwei Zhu,Shengwei Zhu,Xizeng Mao,Jianxun Feng,Yong-Mei Qin,Liang Zhang,Jing Cheng,Liping Wei,Zhi-Yong Wang,Zhi-Yong Wang,Yu-Xian Zhu +11 more
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TLDR
The results indicate that ethylene plays a major role in promoting cotton fiber elongation and may promote cell elongation by increasing the expression of sucrose synthase, tubulin, and expansin genes.Abstract:
Upland cotton (Gossypium hirsutum) produces the most widely used natural fibers, yet the regulatory mechanisms governing fiber cell elongation are not well understood. Through sequencing of a cotton fiber cDNA library and subsequent microarray analysis, we found that ethylene biosynthesis is one of the most significantly upregulated biochemical pathways during fiber elongation. The 1-Aminocyclopropane-1-Carboxylic Acid Oxidase1-3 (ACO1-3) genes responsible for ethylene production were expressed at significantly higher levels during this growth stage. The amount of ethylene released from cultured ovules correlated with ACO expression and the rate of fiber growth. Exogenously applied ethylene promoted robust fiber cell expansion, whereas its biosynthetic inhibitor l-(2-aminoethoxyvinyl)-glycine (AVG) specifically suppressed fiber growth. The brassinosteroid (BR) biosynthetic pathway was modestly upregulated during this growth stage, and treatment with BR or its biosynthetic inhibitor brassinazole (BRZ) also promoted or inhibited, respectively, fiber growth. However, the effect of ethylene treatment was much stronger than that of BR, and the inhibitory effect of BRZ on fiber cells could be overcome by ethylene, but the AVG effect was much less reversed by BR. These results indicate that ethylene plays a major role in promoting cotton fiber elongation. Furthermore, ethylene may promote cell elongation by increasing the expression of sucrose synthase, tubulin, and expansin genes.read more
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KOBAS 2.0: a web server for annotation and identification of enriched pathways and diseases
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Genome sequence of cultivated Upland cotton ( Gossypium hirsutum TM-1) provides insights into genome evolution
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Book
Biochemistry & Molecular Biology of Plants
TL;DR: This edition of Biochemistry & Molecular Biology of Plants holds a unique place in the plant sciences literature as it provides the only comprehensive, authoritative, integrated single volume book in this essential field of study.
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