Transgenic Mouse Proteomics Identifies New 14-3-3-associated Proteins Involved in Cytoskeletal Rearrangements and Cell Signaling
Pierre-Olivier Angrand,Inmaculada Segura,Pamela Völkel,Sonja Ghidelli,Rebecca Terry,Miro Brajenovic,Kristina Vintersten,Rüdiger Klein,Giulio Superti-Furga,Gerard Drewes,Bernhard Kuster,Tewis Bouwmeester,Amparo Acker-Palmer +12 more
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TLDR
This study describes, for the first time, the application of the tandem affinity purification (TAP) and LC-MS method to the characterization of protein complexes from transgenic mice, allowing the identification of almost 40 novel 14-3-3ζ-binding proteins.About:
This article is published in Molecular & Cellular Proteomics.The article was published on 2006-12-01 and is currently open access. It has received 147 citations till now. The article focuses on the topics: GTPase-activating protein & Cell signaling.read more
Citations
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Journal ArticleDOI
The E3 Ligase MuRF1 degrades myosin heavy chain protein in dexamethasone-treated skeletal muscle.
Brian A. Clarke,Doreen Drujan,Monte S. Willis,Leon Murphy,Richard A. Corpina,Elena Burova,Sergey V. Rakhilin,Trevor Stitt,Cam Patterson,Esther Latres,David J. Glass +10 more
TL;DR: The mechanism by which MYH is depleted under atrophy conditions is identified and it is demonstrated that inhibition of a single E3 ligase, MuRF1, is sufficient to maintain this important sarcomeric protein.
Journal ArticleDOI
Methods for the detection and analysis of protein-protein interactions
TL;DR: Four strategies for validation of protein–protein interactions are presented: confocal microscopy for intracellular colocalization of proteins, coimmunoprecipitation, surface plasmon resonance (SPR) and spectroscopic studies.
Journal ArticleDOI
Affinity-purification coupled to mass spectrometry: basic principles and strategies.
TL;DR: The basic principles used in affinity‐purification coupled to mass spectrometry are reviewed, with an emphasis on tools (both biochemical and computational), which enable the discovery and reporting of high quality protein–protein interactions.
Journal ArticleDOI
Targeted tandem affinity purification of PSD-95 recovers core postsynaptic complexes and schizophrenia susceptibility proteins
Esperanza Fernández,Mark O. Collins,Rachel T Uren,Maksym V. Kopanitsa,Noboru H. Komiyama,Mike D. R. Croning,Lysimachos Zografos,J. Douglas Armstrong,Jyoti S. Choudhary,Seth G. N. Grant +9 more
TL;DR: This work combines mouse genetics and proteomics to characterize synapse protein complexes and interaction networks, andnotation of clusters with human disease associations revealed that multiple disorders map to the network, with a significant correlation of schizophrenia within the glutamate receptor clusters.
Journal ArticleDOI
Identification of piRNAs in the central nervous system
Eun Joo Lee,Sourav Banerjee,Hongjun Zhou,Aruna Jammalamadaka,Mary Luz Arcila,B.S. Manjunath,Kenneth S. Kosik +6 more
TL;DR: A more widespread expression of a limited set of piRNAs is reported and particularly focus on their expression in the hippocampus, which suggested a role in spine morphogenesis.
References
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Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels
TL;DR: Silver staining allows a substantial shortening of sample preparation time and may, therefore, be preferable over Coomassie staining, and this work removes a major obstacle to the low-level sequence analysis of proteins separated on polyacrylamide gels.
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Probability-based protein identification by searching sequence databases using mass spectrometry data.
TL;DR: A new computer program, Mascot, is presented, which integrates all three types of search for protein identification by searching a sequence database using mass spectrometry data, and the scoring algorithm is probability based.
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A generic protein purification method for protein complex characterization and proteome exploration.
Guillaume Rigaut,Anna Shevchenko,Berthold Rutz,Matthias Wilm,Matthias Mann,Bertrand Séraphin +5 more
TL;DR: A generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag and Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein.
Journal ArticleDOI
Serine Phosphorylation of Death Agonist BAD in Response to Survival Factor Results in Binding to 14-3-3 Not BCL-XL
TL;DR: The rapid phosphorylation of BAD following IL-3 connects a proximal survival signal with the BCL-2 family, modulating this checkpoint for apoptosis and enhanced BAD's death-promoting activity.
Journal ArticleDOI
The structural basis for 14-3-3:phosphopeptide binding specificity.
Michael B. Yaffe,Katrin Rittinger,Stefano Volinia,Paul R. Caron,Alastair Aitken,Henrik Leffers,Steven J. Gamblin,Stephen J. Smerdon,Lewis C. Cantley,Lewis C. Cantley +9 more
TL;DR: It is shown that the 14-3-3 dimer binds tightly to single molecules containing tandem repeats of phosphoserine motifs, implicating bidentate association as a signaling mechanism with molecules such as Raf, BAD, and Cbl.
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