Translation and codon usage regulate Argonaute slicer activity to trigger small RNA biogenesis.
Meetali Singh,Eric Cornes,Blaise Li,Piergiuseppe Quarato,Piergiuseppe Quarato,Loan Bourdon,Florent Dingli,Damarys Loew,Simone Proccacia,Simone Proccacia,Germano Cecere +10 more
TLDR
In this paper, it was shown that CSR-1 slicer activity is primarily involved in triggering the synthesis of small RNAs on the coding sequences of germline mRNAs and posttranscriptionally regulates a fraction of targets.Abstract:
In the Caenorhabditis elegans germline, thousands of mRNAs are concomitantly expressed with antisense 22G-RNAs, which are loaded into the Argonaute CSR-1. Despite their essential functions for animal fertility and embryonic development, how CSR-1 22G-RNAs are produced remains unknown. Here, we show that CSR-1 slicer activity is primarily involved in triggering the synthesis of small RNAs on the coding sequences of germline mRNAs and post-transcriptionally regulates a fraction of targets. CSR-1-cleaved mRNAs prime the RNA-dependent RNA polymerase, EGO-1, to synthesize 22G-RNAs in phase with translating ribosomes, in contrast to other 22G-RNAs mostly synthesized in germ granules. Moreover, codon optimality and efficient translation antagonize CSR-1 slicing and 22G-RNAs biogenesis. We propose that codon usage differences encoded into mRNA sequences might be a conserved strategy in eukaryotes to regulate small RNA biogenesis and Argonaute targeting.read more
Citations
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Emerging roles and functional mechanisms of PIWI-interacting RNAs
TL;DR: The most recent advances in the understanding of piRNA biogenesis, the molecular mechanisms of pi RNA function and the emerging roles of piRNAs in germ line development mainly in flies and mice, and in infertility, cancer and neurological diseases in humans are discussed.
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piRNAs initiate transcriptional silencing of spermatogenic genes during C. elegans germline development
TL;DR: In this paper , the authors show that C. elegans piRNAs trigger the transcriptional silencing of hundreds of spermatogenic genes during sperminogenesis, promoting sperm differentiation and function.
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Small RNAs in epigenetic inheritance: from mechanisms to trait transmission
TL;DR: The role of small RNAs in transmitting epigenetic information across generations in animals is discussed in this paper, focusing on the mechanisms by which smallRNAs achieve such a function, from the silencing of transgenes to the inheritance of complex traits, such as fertility, stress responses, infections, and behavior.
Journal ArticleDOI
Germ granules and gene regulation in the Caenorhabditis elegans germline.
TL;DR: An overview of germ granules is provided and the significance of more recent observations that describe how they further demix into sub-granules are focused on, each with unique compositions and functions.
Journal ArticleDOI
Mechanisms of epigenetic regulation by C. elegans nuclear RNA interference pathways
Arneet L. Saltzman,Naliyah Kaya +1 more
TL;DR: In this article , the authors highlight recent discoveries in the field of nuclear RNA interference in C. elegans and the roles of nuclear AGO/sRNA complexes in the regulation of gene expression, chromatin organization, genome stability, and transgenerational epigenetic inheritance.
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