Journal ArticleDOI
Translation of Polarity Cues into Asymmetric Spindle Positioning in Caenorhabditis elegans Embryos.
Kelly Colombo,Stephan W. Grill,Randall J. Kimple,Francis S. Willard,David P. Siderovski,Pierre Gönczy +5 more
TLDR
The extent of net pulling forces may depend on cortical Gα activity, which is regulated by anterior-posterior polarity cues through GPR-1/2, which was found to interact with guanosine diphosphate-bound GOA-1 and were enriched on the posterior cortex in a par-3– and par-2–dependent manner.Abstract:
Asymmetric divisions are crucial for generating cell diversity; they rely on coupling between polarity cues and spindle positioning, but how this coupling is achieved is poorly understood. In one-cell stage Caenorhabditis elegans embryos, polarity cues set by the PAR proteins mediate asymmetric spindle positioning by governing an imbalance of net pulling forces acting on spindle poles. We found that the GoLoco-containing proteins GPR-1 and GPR-2, as well as the Galpha subunits GOA-1 and GPA-16, were essential for generation of proper pulling forces. GPR-1/2 interacted with guanosine diphosphate-bound GOA-1 and were enriched on the posterior cortex in a par-3- and par-2-dependent manner. Thus, the extent of net pulling forces may depend on cortical Galpha activity, which is regulated by anterior-posterior polarity cues through GPR-1/2.read more
Citations
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Mechanical forces direct stem cell behaviour in development and regeneration
Kyle H. Vining,David J. Mooney +1 more
TL;DR: Fundamental insights into the mechanobiology of stem cells also inform the design of artificial niches to support stem cells for regenerative therapies.
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Mechanical control of tissue and organ development
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The PAR Proteins: Fundamental Players in Animal Cell Polarization
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TL;DR: The par genes were discovered in genetic screens for regulators of cytoplasmic partitioning in the early embryo of C. elegans, and encode six different proteins required for asymmetric cell division by the worm zygote.
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Asymmetric cell division: recent developments and their implications for tumour biology
TL;DR: This work has shown that localized phosphorylation events are responsible for the asymmetric segregation of cell fate determinants in mitosis and that centrosomes and microtubules play important parts in this process.
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Survey of the year 2003 commercial optical biosensor literature
Rebecca L. Rich,David G. Myszka +1 more
TL;DR: In this overview, 13 papers that should be on everyone's ‘must read’ list for 2003 are spotlighted and examples of how to identify and interpret high‐quality biosensor data are provided.
References
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Journal ArticleDOI
The embryonic cell lineage of the nematode Caenorhabditis elegans.
TL;DR: It is concluded that the cell lineage itself, complex as it is, plays an important role in determining cell fate and is demonstrated to demonstrate substantial cell autonomy in at least some sections of embryogenesis.
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Post-embryonic cell lineages of the nematode, Caenorhabditis elegans
John Sulston,H.R. Horvitz +1 more
TL;DR: These cell lineages range in length from one to eight sequential divisions and lead to significant developmental changes in the neuronal, muscular, hypodermal, and digestive systems and are determined by direct observation of the divisions, migrations, and deaths of individual cells in living nematodes.
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Specific interference by ingested dsRNA
Lisa Timmons,Andrew Fire +1 more
TL;DR: This work shows that C. elegans can respond in a gene-specific manner to dsRNA encountered in the environment, and finds that Escherichia coli bacteria expressing dsRNAs can confer specific interference effects on the nematode larvae that feed on them.
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Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans
TL;DR: An efficient induction of RNAi using bacteria to deliver double-stranded RNA is reported, found to be most effective in inducing RNAi for non-neuronal tissue of late larval and adult hermaphrodites, with decreased effectiveness in the nervous system, in early larval stages, and in males.