Chad Nusbaum

TL;DR: The Hi-C protocol can identify genomic loci that are spatially co-located in vivo as discussed by the authors, which may include intrachromosomal interactions and/or interchromosomal interaction.

TL;DR: In the version of this article initially published, the fourth sentence in the Online Methods section “RNA extraction and library preparation,” that read in part “procedure that combines a random priming step with a shearing step and results in fragments of ∼700 bp in size,’” should have read, ”procedures that combines fragmentation of mRNA to a peak size of ∼750 nucleotides by heating6 followed by random-primed reverse transcription

TL;DR: The utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP- sequencing on the Ion Torrent platform is demonstrated.

TL;DR: In this paper, the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) was demonstrated.

TL;DR: Analysis of kidney samples from lupus nephritis patients and healthy controls revealed 21 subsets of leukocytes active in disease, including multiple populations of myeloid, T, NK and B cells, demonstrating both pro-inflammatory and resolving responses.