H
Helge Ewers
Researcher at Free University of Berlin
Publications - 87
Citations - 5053
Helge Ewers is an academic researcher from Free University of Berlin. The author has contributed to research in topics: Microscopy & Super-resolution microscopy. The author has an hindex of 29, co-authored 75 publications receiving 4255 citations. Previous affiliations of Helge Ewers include École Polytechnique Fédérale de Lausanne & King's College London.
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Journal ArticleDOI
A simple, versatile method for GFP-based super-resolution microscopy via nanobodies
TL;DR: This work developed a method to use any GFP-tagged construct in single-molecule super-resolution microscopy by targeting GFP with small, high-affinity antibodies coupled to organic dyes and achieved nanometer spatial resolution and minimal linkage error when analyzing microtubules, living neurons and yeast cells.
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GM1 structure determines SV40-induced membrane invagination and infection
Helge Ewers,Winfried Römer,Winfried Römer,Alicia E. Smith,Kirsten Bacia,Kirsten Bacia,Serge Dmitrieff,Wengang Chai,Roberta Mancini,Jürgen Kartenbeck,Jürgen Kartenbeck,Valérie Chambon,Valérie Chambon,Ludwig Berland,Ludwig Berland,Ariella Oppenheim,Günter Schwarzmann,Ten Feizi,Petra Schwille,Pierre Sens,Ari Helenius,Ludger Johannes,Ludger Johannes +22 more
TL;DR: This analysis indicates that SV40, other polyoma viruses and some bacterial toxins use glycosphingolipids and a common pentameric protein scaffold to induce plasma membrane curvature, thus directly promoting their endocytic uptake into cells.
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High-speed nanoscopic tracking of the position and orientation of a single virus.
TL;DR: A colocalization methodology that combines scattering interferometry and single-molecule fluorescence microscopy to visualize both position and orientation of single quantum dot–labeled Simian virus 40 (SV40) particles suggests recurrent swap of receptors and viral pentamers as well as receptor aggregation in nanodomains.
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The 2015 super-resolution microscopy roadmap
Stefan W. Hell,Stefan W. Hell,Steffen J. Sahl,Mark Bates,Xiaowei Zhuang,Rainer Heintzmann,Rainer Heintzmann,Rainer Heintzmann,Martin J. Booth,Joerg Bewersdorf,Gleb Shtengel,Harald F. Hess,Philip Tinnefeld,Alf Honigmann,Stefan Jakobs,Ilaria Testa,Laurent Cognet,Brahim Lounis,Helge Ewers,Simon J. Davis,Christian Eggeling,David Klenerman,Katrin I. Willig,Giuseppe Vicidomini,Marco Castello,Alberto Diaspro,Thorben Cordes +26 more
TL;DR: Super-resolution optical microscopy is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study of living cells and organisms.
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Single-particle tracking of murine polyoma virus-like particles on live cells and artificial membranes.
TL;DR: The results suggested that clustering of ganglioside molecules by the multivalent VLPs induced transmembrane coupling that led to confinement of the virus/receptor complex by cortical actin filaments.