M
Manuela Wissler
Researcher at University of Freiburg
Publications - 22
Citations - 5049
Manuela Wissler is an academic researcher from University of Freiburg. The author has contributed to research in topics: Severe combined immunodeficiency & Genetic enhancement. The author has an hindex of 13, co-authored 21 publications receiving 4811 citations. Previous affiliations of Manuela Wissler include National Institutes of Health & University of Washington.
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Journal ArticleDOI
LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1.
Salima Hacein-Bey-Abina,C von Kalle,C von Kalle,Manfred Schmidt,Matthew P. McCormack,NM Wulffraat,Philippe Leboulch,Annick Lim,Cameron S. Osborne,R. Pawliuk,Estelle Morillon,R. Sorensen,A. Forster,Peter Fraser,Jeffrey I. Cohen,G de Saint Basile,Ian E. Alexander,Uwe Wintergerst,Thierry Frebourg,Alain Aurias,Dominique Stoppa-Lyonnet,Serge Romana,I. Radford-Weiss,Fabian Gross,Françoise Valensi,Eric Delabesse,Elizabeth Macintyre,F. Sigaux,Jean Soulier,L. E. Leiva,Manuela Wissler,Claudia Prinz,Terence H. Rabbitts,F. Le Deist,Alain Fischer,Marina Cavazzana-Calvo +35 more
TL;DR: Retrovirus vector insertion can trigger deregulated premalignant cell proliferation with unexpected frequency, most likely driven by retrovirus enhancer activity on the LMO2 gene promoter.
Journal ArticleDOI
Vector integration is nonrandom and clustered and influences the fate of lymphopoiesis in SCID-X1 gene therapy
Annette Deichmann,Salima Hacein-Bey-Abina,Manfred Schmidt,Manfred Schmidt,Alexandrine Garrigue,Martijn H. Brugman,Jingqiong Hu,Hanno Glimm,Gabor Gyapay,Bernard Prum,Christopher Fraser,Nicolas Fischer,Kerstin Schwarzwaelder,Kerstin Schwarzwaelder,Maria Luise Siegler,Dick de Ridder,Dick de Ridder,Karin Pike-Overzet,Steven J. Howe,Adrian J. Thrasher,Gerard Wagemaker,Ulrich Abel,Frank J. T. Staal,Eric Delabesse,Jean-Luc Villeval,Bruce J. Aronow,Christophe Hue,Claudia Prinz,Manuela Wissler,Chuck Klanke,Jean Weissenbach,Ian E. Alexander,Alain Fischer,Christof von Kalle,Marina Cavazzana-Calvo +34 more
TL;DR: The results obtained from a large-scale mapping of retroviral integration sites isolated from cells of 9 patients with X-linked SCID (SCID-X1) treated with a retrovirus-based gene therapy protocol help to elucidate the relationship between vector insertion and long-term in vivo selection of transduced cells in human patients with SCID- X1.
Journal ArticleDOI
Polyclonal long-term repopulating stem cell clones in a primate model.
Manfred Schmidt,Manfred Schmidt,Manfred Schmidt,Philipp Zickler,Philipp Zickler,Philipp Zickler,Gesa Hoffmann,Gesa Hoffmann,Gesa Hoffmann,Sebastian Haas,Sebastian Haas,Sebastian Haas,Manuela Wissler,Manuela Wissler,Manuela Wissler,Arne Muessig,Arne Muessig,Arne Muessig,John F. Tisdale,John F. Tisdale,John F. Tisdale,Ken Kuramoto,Ken Kuramoto,Ken Kuramoto,Robert G. Andrews,Robert G. Andrews,Robert G. Andrews,Tong Wu,Tong Wu,Tong Wu,Hans-Peter Kiem,Hans-Peter Kiem,Hans-Peter Kiem,Cynthia E. Dunbar,Cynthia E. Dunbar,Cynthia E. Dunbar,Christof von Kalle +36 more
TL;DR: This study provides direct molecular evidence for a polyclonal, multilineage, and sustained contribution of individual stem cells to primate hematopoiesis and demonstrates the derivation of circulating peripheral blood cells from individual stem cell clones.
Journal ArticleDOI
Gammaretrovirus-mediated correction of SCID-X1 is associated with skewed vector integration site distribution in vivo
Kerstin Schwarzwaelder,Steven J. Howe,Manfred Schmidt,Manfred Schmidt,Martijn H. Brugman,Annette Deichmann,Annette Deichmann,Hanno Glimm,Hanno Glimm,Sonja Schmidt,Claudia Prinz,Manuela Wissler,Douglas King,Fang Zhang,Kathryn L. Parsley,Kimberly Gilmour,Joanna Sinclair,Jinhua Bayford,Rachel Peraj,Karin Pike-Overzet,Frank J. T. Staal,Dick de Ridder,Dick de Ridder,Christine Kinnon,Ulrich Abel,Gerard Wagemaker,H. Bobby Gaspar,Adrian J. Thrasher,Christof von Kalle +28 more
TL;DR: The divergence of RIS target frequency between transduced progenitor cells and post-thymic T lymphocytes indicates that vector integration influences cell survival, engraftment, or proliferation.
Journal ArticleDOI
Detection and direct genomic sequencing of multiple rare unknown flanking DNA in highly complex samples.
Manfred Schmidt,C von Kalle,Gesa Hoffmann,Manuela Wissler,N. Lemke,A. Müßig,H. Glimm,H. Glimm,D. A. Williams,D. A. Williams,Susanne Ragg,C. U. Hesemann +11 more
TL;DR: EpTS/LM-PCR enables studies of retro- and lentiviral integration, viral vector tracking in gene therapy, insertional mutagenesis, transgene integration, and direct genomic sequencing that until now have been difficult or impossible to perform.