抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A

THE DESIGN AND ANALYSIS OF EXPERIMENTS. By Oscar Kempthorne. New York, John Wiley and Sons, Inc., 1952. 631 pp. $8.50. This book by a teacher of statistics (as well as a consultant for \"experimenters\") is a comprehensive study of the philosophical background for the statistical design of experiment. It is necessary to have some facility with algebraic notation and manipulation to be able to use the volume intelligently. The problems are presented from the theoretical point of view, without such practical examples as would be helpful for those not acquainted with mathematics. The mathematical justification for the techniques is given. As a somewhat advanced treatment of the design and analysis of experiments, this volume will be interesting and helpful for many who approach statistics theoretically as well as practically. With emphasis on the \"why,\" and with description given broadly, the author relates the subject matter to the general theory of statistics and to the general problem of experimental inference. MARGARET J. ROBERTSON

The Design and Analysis of Experiments

The 11th edition of Harrison's Principles of Internal Medicine welcomes Anthony Fauci to its editorial staff, in addition to more than 85 new contributors. While the organization of the book is similar to previous editions, major emphasis has been placed on disorders that affect multiple organ systems. Important advances in genetics, immunology, and oncology are emphasized. Many chapters of the book have been rewritten and describe major advances in internal medicine. Subjects that received only a paragraph or two of attention in previous editions are now covered in entire chapters. Among the chapters that have been extensively revised are the chapters on infections in the compromised host, on skin rashes in infections, on many of the viral infections, including cytomegalovirus and Epstein-Barr virus, on sexually transmitted diseases, on diabetes mellitus, on disorders of bone and mineral metabolism, and on lymphadenopathy and splenomegaly. The major revisions in these chapters and many

Harrison's Principles of Internal Medicine

Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications

Rapid growth in nanotechnology is increasing the likelihood of engineered nanomaterials coming into contact with humans and the environment. Nanoparticles interacting with proteins, membranes, cells, DNA and organelles establish a series of nanoparticle/biological interfaces that depend on colloidal forces as well as dynamic biophysicochemical interactions. These interactions lead to the formation of protein coronas, particle wrapping, intracellular uptake and biocatalytic processes that could have biocompatible or bioadverse outcomes. For their part, the biomolecules may induce phase transformations, free energy releases, restructuring and dissolution at the nanomaterial surface. Probing these various interfaces allows the development of predictive relationships between structure and activity that are determined by nanomaterial properties such as size, shape, surface chemistry, roughness and surface coatings. This knowledge is important from the perspective of safe use of nanomaterials.

Understanding biophysicochemical interactions at the nano–bio interface

Surface instabilities during swelling of pH-sensitive hydrogels

Kinetics of mucus-polymer interactions

(1995). Bioadhesives for Optimization of Drug Delivery. Journal of Drug Targeting: Vol. 3, No. 3, pp. 183-184.

Bioadhesives for Optimization of Drug Delivery

Poly[(diethylaminoethyl methacrylate)-graft-(ethylene glycol)] hydrogels were prepared with a molar ratio of 10:1 of diethylaminoethyl methacrylate to poly(ethylene glycol) of number-average molecular weights (Mn) 200, 400 and 1000 g mol−1 using tetra(ethylene glycol) dimethacrylate to give a crosslinking ratio between 0.5 and 4.0 %. Glucose oxidase and catalase were immobilized in the matrix during polymerization. The maximum enzyme loading used was 6.6 × 10−4 g of glucose oxidase per g of polymer. The equilibrium and dynamic swelling properties of these hydrogels were investigated. The pH-dependent equilibrium swelling characteristics showed a sharp transition between the swollen and the collapsed state at pH 7.0. The dynamic response of the hydrogel discs to pH was analyzed in pulsatile pH conditions. The effects of particle size, crosslinking and molecular weight of poly(ethylene glycol) (PEG) on the dynamic swelling response were investigated. The pulsatile nature of the response was analyzed using Boltzmann superposition. Swelling–pH master curves were obtained. Copyright © 2004 Society of Chemical Industry

Relaxational behavior and swelling‐pH master curves of poly[(diethylaminoethyl methacrylate)‐graft‐(ethylene glycol)] hydrogels

The emerging field of regenerative engineering offers a great challenge and an even greater opportunity for materials scientists and engineers. How can we develop materials that are highly porous to permit cellular infiltration, yet possess sufficient mechanical integrity to mimic native tissues? How can we retain and deliver bioactive molecules to drive cell organization, proliferation, and differentiation in a predictable manner? In the following perspective, we highlight recent studies that have demonstrated the vital importance of each of these questions, as well as many others pertaining to scaffold development. We posit hybrid materials synthesized by molecular decoration and molecular imprinting as intelligent biomaterials for regenerative engineering applications. These materials have potential to present cell adhesion molecules and soluble growth factors with fine-tuned spatial and temporal control, in response to both cell-driven and external triggers. Future studies in this area will address a pertinent clinical need, expand the existing repertoire of medical materials, and improve the field’s understanding of how cells and materials respond to one another. Regenerative engineering seeks to combine our growing understandings of materials, stem cells, and developmental biology to generate therapeutic and curative treatments for a range of diseases. In this perspective, we discuss the utility and limitations of existing materials employed for regenerative engineering applications. These materials balance the dynamic need to provide mechanical strength, present therapeutic biomolecules, permit cell entry, and degrade over time. Then, we present recent developments in the field of materials science, which have generated hybrids of natural and synthetic origin. These blended, conjugated, and/or functionalized materials engage in intelligent and responsive interactions with the biological host. Specific interaction-response examples are discussed for the regeneration of nerve, bone, and cardiac muscle. In the future, intelligent materials for regenerative engineering will respond dynamically to signals produced by a patient’s cells or administered in a clinical intervention to facilitate tissue growth, healing, and recovery.

Nicholas A. Peppas

Papers

Surface instabilities during swelling of pH-sensitive hydrogels

Kinetics of mucus-polymer interactions

Bioadhesives for Optimization of Drug Delivery

Relaxational behavior and swelling‐pH master curves of poly[(diethylaminoethyl methacrylate)‐graft‐(ethylene glycol)] hydrogels

Vision for Functionally Decorated and Molecularly Imprinted Polymers in Regenerative Engineering