Peter Messiaen

TL;DR: Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information.

TL;DR: In first-line therapy, integrase inhibitors are superior to other regimens and careful use is however warranted when replacing a high genetic barrier drug in treatment-experienced patients switching successful treatment.

TL;DR: Using ultra-deep sequencing, recently more available and affordable, as a tool for the detection of HIV-1 minority species carrying drug resistant mutation (DRM) in a clinical setting, it could not confirm an association between the presence of low minority variants harbouring RT mutations at the start of therapy and primary or secondary therapeutic failure.

TL;DR: Poisson-based analysis of the Alu-HIV PCR data enables absolute quantification without the need of a standard dilution curve and provides a statistical basis for the required minimal number of technical replicates.

TL;DR: National indicators, with clear targets and incentives, are not commonly used in Europe and there are wide variations between countries regarding the selected indicators, the units of measure and the chosen targets.