Journal ArticleDOI
The need for transparency and good practices in the qPCR literature
Stephen A. Bustin,Vladimir Benes,Jeremy A. Garson,Jan Hellemans,Jim F. Huggett,Mikael Kubista,Reinhold Mueller,Tania Nolan,Michael W. Pfaffl,Gregory L. Shipley,Carl T. Wittwer,Peter Schjerling,Philip J. R. Day,Mónica Abreu,Begoña Aguado,Jean-François Beaulieu,Anneleen Beckers,Sara Bogaert,John A. Browne,Fernando Carrasco-Ramiro,Liesbeth Ceelen,Kate L. Ciborowski,Pieter Cornillie,Stephanie Coulon,Ann Cuypers,Sara De Brouwer,Leentje De Ceuninck,Jurgen De Craene,Hélène De Naeyer,Ward De Spiegelaere,Kato Deckers,Annelies Dheedene,Kaat Durinck,Margarida Ferreira-Teixeira,Annelies Fieuw,Jack M. Gallup,Sandra Gonzalo-Flores,Karen Goossens,Femke Heindryckx,Elizabeth Herring,Hans Hoenicka,Laura Icardi,Rolf Jaggi,Farzad Javad,Michael Karampelias,Frederick S. B. Kibenge,Molly J. T. Kibenge,Candy Kumps,Irina Lambertz,Tim Lammens,Amelia Markey,Peter Messiaen,Evelien Mets,Sofia Morais,Alberto Mudarra-Rubio,Justine K. Nakiwala,Hilde Nelis,Pål A. Olsvik,Claudina Perez-Novo,Michelle Plusquin,Tony Remans,Ali Rihani,Paulo Rodrigues-Santos,Pieter Rondou,Rebecca Sanders,Katharina Schmidt-Bleek,Kerstin Skovgaard,Karen Smeets,Laura Tabera,Stefan Toegel,Tim Van Acker,Wim Van den Broeck,Joni Van der Meulen,Mireille Van Gele,Gert Van Peer,Mario Van Poucke,Nadine Van Roy,Sarah Vergult,Joris Wauman,Marina Tshuikina-Wiklander,Erik Willems,Sara Zaccara,Fjoralba Zeka,Jo Vandesompele +83 more
TLDR
Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information.Abstract:
Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.read more
Citations
More filters
Journal ArticleDOI
Circular RNAs in cancer: opportunities and challenges in the field.
TL;DR: The current knowledge on circRNAs is reviewed in relation to their implications in tumorigenesis as well as their potential as diagnostic and prognostic biomarkers and as possible therapeutic targets in future personalized medicine.
Journal ArticleDOI
EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research
Jan Van Deun,Pieter Mestdagh,Patrizia Agostinis,Özden Akay,Sushma Anand,Jasper Anckaert,Zoraida Andreu Martinez,Tine Baetens,Els Beghein,Laurence Bertier,Geert Berx,Janneke Boere,Stephanie Boukouris,Michel Bremer,Dominik Buschmann,James Brian Byrd,Clara Casert,Lesley Cheng,Anna Cmoch,Delphine Daveloose,Eva De Smedt,Seyma Demirsoy,Victoria Depoorter,Bert Dhondt,Tom A. P. Driedonks,Aleksandra M. Dudek,Abdou ElSharawy,Ilaria Floris,Andrew D Foers,Kathrin Gärtner,Abhishek D. Garg,Edward Geeurickx,Jan Gettemans,Farzaneh Ghazavi,Bernd Giebel,Tom Groot Kormelink,Grace V. Hancock,Hetty Helsmoortel,Andrew F. Hill,Vincent Hyenne,Hina Kalra,David Kim,Joanna Kowal,Joanna Kowal,Sandra Kraemer,Petra Leidinger,Carina Leonelli,Yaxuan Liang,Lien Lippens,Shu Liu,Alessandra Lo Cicero,Alessandra Lo Cicero,Shaun Martin,Suresh Mathivanan,Prabhu Mathiyalagan,Tamás Matusek,Gloria Milani,Marta Monguió-Tortajada,Liselot Mus,Dillon C. Muth,Andrea Németh,Esther N. M. Nolte-‘t Hoen,Lorraine O'Driscoll,Roberta Palmulli,Roberta Palmulli,Michael W. Pfaffl,Bjarke Primdal-Bengtson,Bjarke Primdal-Bengtson,Erminia Romano,Quentin Rousseau,Susmita Sahoo,Natalia G. Sampaio,Monisha Samuel,Benjamin J. Scicluna,Bieke Soen,Anneleen Steels,Johannes V. Swinnen,Maarit Takatalo,Safia Thaminy,Clotilde Théry,Clotilde Théry,Joeri Tulkens,Isabel Van Audenhove,Susanne G. van der Grein,Alan Van Goethem,Martijn J. C. van Herwijnen,Guillaume van Niel,Guillaume van Niel,Nadine Van Roy,Alexander R. van Vliet,Niels Vandamme,Suzanne Vanhauwaert,Glenn Vergauwen,Frederik J. Verweij,Frederik J. Verweij,Annelynn Wallaert,Marca H. M. Wauben,Kenneth W. Witwer,Marijke I. Zonneveld,Olivier De Wever,Jo Vandesompele,An Hendrix +101 more
TL;DR: It is argued that the field of extracellular vesicle (EV) biology needs more transparent reporting to facilitate interpretation and replication of experiments and EV-TRACK, a crowdsourcing knowledgebase that centralizes EV biology and methodology, is described.
Journal ArticleDOI
The Ultimate qPCR Experiment: Producing Publication Quality, Reproducible Data the First Time
TL;DR: The critical pitfalls and sources of error in qPCR experiments are described, along with a rigorous, stepwise process to minimize variability, time, and cost in generating reproducible, publication quality data every time.
Journal ArticleDOI
Considerations for Digital PCR as an Accurate Molecular Diagnostic Tool
TL;DR: Some of the considerations that may be needed when applying dPCR are highlighted and sources of error are discussed to assist in the translation of d PCR to diagnostic, predictive, or prognostic applications.
Journal ArticleDOI
dPCR: A Technology Review.
TL;DR: This review compares the fundamental concepts behind the quantification of nucleic acids by dPCR and quantitative real-time PCR and examines how isothermal amplification could be an alternative to PCR in digital assays.
References
More filters
Journal ArticleDOI
A new mathematical model for relative quantification in real-time RT-PCR.
TL;DR: This study enters into the particular topics of the relative quantification in real-time RT-PCR of a target gene transcript in comparison to a reference gene transcript and presents a new mathematical model that needs no calibration curve.
Journal ArticleDOI
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
Jo Vandesompele,Katleen De Preter,Filip Pattyn,Bruce Poppe,Nadine Van Roy,Anne De Paepe,Franki Speleman +6 more
TL;DR: The normalization strategy presented here is a prerequisite for accurate RT-PCR expression profiling, which opens up the possibility of studying the biological relevance of small expression differences.
Journal ArticleDOI
The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Stephen A. Bustin,Vladimir Benes,Jeremy A. Garson,Jan Hellemans,Jim F. Huggett,Mikael Kubista,Reinhold Mueller,Tania Nolan,Michael W. Pfaffl,Gregory L. Shipley,Jo Vandesompele,Carl T. Wittwer,Carl T. Wittwer +12 more
TL;DR: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency.
Journal ArticleDOI
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.
TL;DR: The technical aspects involved are discussed, conventional and kinetic RT-PCR methods for quantitating gene expression are contrasted, and the usefulness of these assays are illustrated by demonstrating the significantly different levels of transcription between individuals of the housekeeping gene family, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH).