Showing papers in "Enzyme and Microbial Technology in 2012"

TL;DR: Significant differences between true and sham acupuncture indicate that acupuncture is more than a placebo, suggesting that factors in addition to the specific effects of needling are important contributors to the therapeutic effects of acupuncture.

TL;DR: The effect of enzyme to substrate ratio, initial lactose concentration and temperature has been studied for the kinetically controlled reaction of lactose transgalactosylation with Aspergillus oryzae β-Galactosidase, to produce prebiotic galacto-oligosaccharides (GOS).

TL;DR: This study demonstrates that under heterotrophic conditions, A. brasilense promotes the accumulation of carbohydrates in two strains Chlorella spp.

TL;DR: Brain response to acupuncture stimuli encompasses a broad network of regions consistent with not just somatosensory, but also affective and cognitive processing, and the evidence based on meta-analyses confirmed some of these results.

TL;DR: It is demonstrated here that electricity can be produced in an MFC using cellulose as the electron donor source and this MFC system employs a unique microbial ecology in which both the electron donate (cellulose) and the electron acceptor (carbon paper) are insoluble.

TL;DR: Results indicated that both 25 and 50 mg/L salicylic acid (SA) could increase astaxanthin productivity and enhance transcriptional expression of eight carotenoid genes in H. pluvialis, and suggested that SA constitute molecular signals in the network of astrixanthin biosynthesis.

TL;DR: To ensure maximum enzyme activity recovery, enzyme recycling seems to be most effective when short-term rounds of hydrolysis are combined with the recovery of enzymes from both the liquid and the solid phases and potentially enzyme supplementation to replenish lost activity.

TL;DR: A simple and robust system to produce GABA from glucose using the recombinant Corynebacterium glutamicum strain GAD, which expresses GadB, a glutamate decarboxylase encoded by the gadB gene of Escherichia coli W3110 is reported.

TL;DR: This result illustrates a possible strategy for developing metabolically engineered yeast strain for the economical production of resveratrol from cheap amino acids in Saccharomyces cerevisiae.

TL;DR: A high-expression-secretion vector is engineered that directs proteins towards the extracellular medium in two Aspergillii host strains, and the effect of maltose-induced over-expression and protein secretion as well as time and pH-dependent protein stability in the medium are examined.

TL;DR: Commercial enzymes, creatininase (CA), creatinine (CI) from Pseudomonas sp, sarcosine oxidase (SO) from Bacillus sp were co-immobilized onto iron oxide nanoparticles/chitosan-graft-polyaniline (Fe(3)O(4)-NPs/CHIT-g-PANI) composite film electrodeposited on surface of Pt electrode through glutaraldehyde coupling.

TL;DR: Investigation of the efficiency, stability, and reusability of laccase-poly(lactic-co-glycolic acid) (PLGA) nanofiber for diclofenac transformation suggests it may be useful for removingdicl ofenac from aqueous sources and has potential for other commercial applications.

TL;DR: R reverse engineering and site directed mutagenesis methods were able to confirm that the amino acid substitution of the Asp285 is responsible for the increased aldehyde reductase activities by utilizing the additional cofactor NADPH.

TL;DR: Results indicate the potential of AMPLS andAMPLS-G1 as superior ingredients for functional cosmetics and competitive inhibition against tyrosinase.

TL;DR: NQM1 and TKL2 may play a physiological role via an effect on the non-oxidative pentose phosphate pathway in the xylOSE metabolic pathway, although their roles in xylose utilization and fermentation are less important than those of TAL1 andTKL1.

TL;DR: Investigating the underlying brain mechanisms by which the state of mindfulness reduces pain reveals a unique mechanism of pain modulation, comprising increased sensory processing and decreased cognitive control, and is in sharp contrast to established pain modulation mechanisms.

TL;DR: Nanobiotechnologies focusing on the key requirements of signal amplification and pre-concentration for the development of sensitive assays for food-borne pathogen detection in food matrices will be described and evaluated.

TL;DR: Experimental investigation of the effect of water content revealed that the conversion of GC was maximized with no added water, and the addition of surfactant such as Tween 80 increased the GC conversion, which finally reached 96.25% under the optimum condition and with surfactants addition.

TL;DR: All the galactan- and SPPP-derived products promoted the growth of probiotic strains of Bifidobacterium longum and Lactobacillus acidophilus and generally did not support the propagation of Clostridium perfringens in single culture fermentations.

TL;DR: In this article, the authors investigated the sorption of uranium from aqueous solution onto the magnetically modified yeast cell, Rhodotorula glutinis, in a batch system.

TL;DR: A copolymerized boronate cryogel column was developed for the separation of RNA from the bacterial crude extract without any pre-processing and it was found that the adherent cells can be recovered at relatively higher fructose concentration than non-adherent cells which could be recovered by using low fructose concentration.

TL;DR: This novel yeast may be useful for reducing the cost of bio-ethanol production and may be suitable for industrial-scale bio-Ethanol production.

TL;DR: To restore glucose utilization, overexpression of nicotinic acid phosphoribosyltransferase, a rate limiting enzyme of NAD(H) synthesis encoded by the pncB gene, resulted in a significant increase in cell mass and succinic acid production, providing further evidence that regulation of NAD (H) pool and NADH/NAD(+) ratio was very important for succinic Acid production.

TL;DR: Results suggest that the thermostable β-glucosidase Bgl-gs1 is a likely candidate for industrial applications.

TL;DR: The bioavailability of the nitrogen sources in the novel medium was confirmed in fed-batch bioreactor studies, in which 82.3g dry cell L⁻¹ of the oleaginous yeast Rhodosporidium toruloides Y4 was obtained with a lipid content of 48%.

TL;DR: Three ferulic acid esterases from the filamentous fungus Chrysosporium lucknowense C1 were purified and characterized and are the first members of the phylogenetic subfamily 5 to be biochemically characterized.

TL;DR: Good correlation between results in case of analysis of real samples and good analytical performance of presented glucose biosensor allows to use presented concept for mass production and commercial use.

TL;DR: This system, which combines three types of yeast cells, each displaying T. aurantiacus EGI, T. reesei CBHII and Aspergillus aculeatus BGLI, can be easily optimized by adjusting the combination ratio of each cell type, suggesting the usefulness of this system for cellulosic ethanol production.

TL;DR: These novel compounds could be used to further expand the industrial applications of astragalin glucosides, in particular in the cosmetics industry.

TL;DR: This is the first report on the characterization of a recombinant H(2)O-forming Nox with high activity and thermostability, and could prove to be of interest in industrial applications such as NAD(+) regeneration.