A Comprehensive Subcellular Atlas of the Toxoplasma Proteome via hyperLOPIT Provides Spatial Context for Protein Functions
Konstantin Barylyuk,Ludek Koreny,Huiling Ke,Simon Butterworth,Oliver M. Crook,Imen Lassadi,Vipul Gupta,Eelco C. Tromer,Tobias Mourier,Tim J. Stevens,Lisa M. Breckels,Arnab Pain,Arnab Pain,Arnab Pain,Kathryn S. Lilley,Ross F. Waller +15 more
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TLDR
This work determines the steady-state subcellular location of thousands of proteins simultaneously within the globally prevalent apicomplexan parasite Toxoplasma gondii, and these data reveal the spatial organizations of protein expression and function, adaptation to hosts, and the underlying evolutionary trajectories of these pathogens.About:
This article is published in Cell Host & Microbe.The article was published on 2020-11-11 and is currently open access. It has received 158 citations till now.read more
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Density-based clustering based on hierarchical density estimates
TL;DR: In this article, the authors proposed a hierarchical density-based hierarchical clustering method, which provides a clustering hierarchy from which a simplified tree of significant clusters can be constructed, and demonstrated that their approach outperforms the current, state-of-the-art, densitybased clustering methods.
A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes
Saima M. Sidik,Diego Huet,My Hang Huynh,Prathapan Thiru,Jeroen P. J. Saeij,Vern B. Carruthers,Sebastian Lourido,Suresh M. Ganesan,Armiyaw S. Nasamu,Jacquin C. Niles,Timothy C. Wang +10 more
TL;DR: The first genome-wide genetic screen of an apicomplexan parasite is presented, revealing essential functions during infection of human cells and providing broad-based functional information on T. gondii genes that will facilitate future approaches to expand the horizon of antiparasitic interventions.
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Molecular characterization of the conoid complex in Toxoplasma reveals its conservation in all apicomplexans, including Plasmodium species.
Ludek Koreny,Mohammad Zeeshan,Konstantin Barylyuk,Eelco C. Tromer,Jolien J. E. van Hooff,Declan Brady,Huiling Ke,Sara Chelaghma,David J. P. Ferguson,David J. P. Ferguson,Laura Eme,Rita Tewari,Ross F. Waller +12 more
TL;DR: The conoid is a conspicuous feature of the apical complex used by apicomplexan parasites, and the conoid proteins have previously been identified, making it difficult to address how conserved this feature is throughout the phylum and whether it is genuinely missing from some major groups as discussed by the authors.
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Biogenesis and discharge of the rhoptries: Key organelles for entry and hijack of host cells by the Apicomplexa.
TL;DR: In this article, the authors review the recent studies on Toxoplasma and Plasmodium parasites that shed light on the key steps leading to rhoptry biogenesis, trafficking, and discharge.
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Composition and stage dynamics of mitochondrial complexes in Plasmodium falciparum.
Felix Evers,Alfredo Cabrera-Orefice,Dei M. Elurbe,Mariska Kea-te Lindert,Sylwia D. Boltryk,Sylwia D. Boltryk,Till S. Voss,Till S. Voss,Martijn A. Huynen,Ulrich Brandt,Ulrich Brandt,Taco W. A. Kooij +11 more
TL;DR: This article applied complexome profiling to map the inventory of protein complexes across the pathogenic asexual blood stages and the transmissible gametocyte stages of Plasmodium falciparum and found that respiratory chain complex components and linked metabolic pathways are up to 40 times more prevalent in gametocytes, while glycolytic enzymes are substantially reduced.
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