A Two-Step Model for de novo Activation of WUSCHEL during Plant Shoot Regeneration
Tian-Qi Zhang,Tian-Qi Zhang,Heng Lian,Chuan-Miao Zhou,Lin Xu,Yuling Jiao,Jiawei Wang,Jiawei Wang +7 more
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TLDR
The results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS, the homeodomain transcription factor for Arabidopsis thaliana.Abstract:
Plant cells are totipotent and competent to regenerate from differentiated organs. It has been known for six decades that cytokinin-rich medium induces shoot regeneration from callus cells. However, the underlying molecular mechanism remains elusive. The homeodomain transcription factor WUSCHEL (WUS) is essential for de novo establishment of the shoot stem cell niche in Arabidopsis thaliana We found that WUS-positive (WUS+) cells mark the shoot progenitor region during regeneration. A cytokinin-rich environment initially promotes the removal of the repressive histone mark H3K27me3 at the WUS locus in a cell cycle-dependent manner. Subsequently, the B-type ARABIDOPSIS RESPONSE REGULATORs (ARRs) ARR1, ARR2, ARR10, and ARR12, which function as transcriptional activators in the cytokinin signaling pathway, spatially activate WUS expression through binding with microRNA165/6-targeted HD-ZIP III transcription factors. Thus, our results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS.read more
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Cytokinin signaling in plant development.
TL;DR: The current understanding of cytokinin metabolism, transport and signaling is summarized, and how this phytohormone regulates changes in gene expression to mediate its pleiotropic effects is discussed.
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A Single-Cell RNA Sequencing Profiles the Developmental Landscape of Arabidopsis Root.
Tian-Qi Zhang,Zhou-Geng Xu,Guan-Dong Shang,Guan-Dong Shang,Jiawei Wang,Jiawei Wang,Jiawei Wang +6 more
TL;DR: This study finds that the cells in Arabidopsis root are highly heterogeneous in their transcriptomes and identifies the expression signatures of intermediate states during root cell differentiation at single-cell resolution.
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Cytokinin action in response to abiotic and biotic stresses in plants.
Anne Cortleven,Jan Erik Leuendorf,Manuel Frank,Daniela Pezzetta,Sylvia Bolt,Thomas Schmülling +5 more
TL;DR: The phytohormone cytokinin was originally discovered as a regulator of cell division and later was described to be involved in regulating numerous processes in plant growth and development including meristem activity, tissue patterning, and organ size.
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Signaling Overview of Plant Somatic Embryogenesis.
Hugo A. Méndez-Hernández,Maharshi Ledezma-Rodríguez,Randy N. Avilez-Montalvo,Yary L. Juárez-Gómez,Analesa Skeete,Johny R. Avilez-Montalvo,Clelia De-la-Peña,Víctor M. Loyola-Vargas +7 more
TL;DR: The role of signaling is examined from the start of cell differentiation through the early steps on the embryogenic pathway, as well as its relation to a plant’s tolerance of different types of stress.
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Cytokinin : a developing story
Brecht Wybouw,Bert De Rybel +1 more
TL;DR: A comprehensive overview of recent findings on how cytokinin influences growth and development in plants is given, and areas for future research are highlighted.
References
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TL;DR: This work shows that the establishment of axillary meristems is regulated by two classical phytohormones, with auxin suppressing and cytokinins promoting axillaryMeristem initiation.