A Two-Step Model for de novo Activation of WUSCHEL during Plant Shoot Regeneration
Tian-Qi Zhang,Tian-Qi Zhang,Heng Lian,Chuan-Miao Zhou,Lin Xu,Yuling Jiao,Jiawei Wang,Jiawei Wang +7 more
Reads0
Chats0
TLDR
The results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS, the homeodomain transcription factor for Arabidopsis thaliana.Abstract:
Plant cells are totipotent and competent to regenerate from differentiated organs. It has been known for six decades that cytokinin-rich medium induces shoot regeneration from callus cells. However, the underlying molecular mechanism remains elusive. The homeodomain transcription factor WUSCHEL (WUS) is essential for de novo establishment of the shoot stem cell niche in Arabidopsis thaliana We found that WUS-positive (WUS+) cells mark the shoot progenitor region during regeneration. A cytokinin-rich environment initially promotes the removal of the repressive histone mark H3K27me3 at the WUS locus in a cell cycle-dependent manner. Subsequently, the B-type ARABIDOPSIS RESPONSE REGULATORs (ARRs) ARR1, ARR2, ARR10, and ARR12, which function as transcriptional activators in the cytokinin signaling pathway, spatially activate WUS expression through binding with microRNA165/6-targeted HD-ZIP III transcription factors. Thus, our results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS.read more
Citations
More filters
Journal ArticleDOI
Cytokinin signaling in plant development.
TL;DR: The current understanding of cytokinin metabolism, transport and signaling is summarized, and how this phytohormone regulates changes in gene expression to mediate its pleiotropic effects is discussed.
Journal ArticleDOI
A Single-Cell RNA Sequencing Profiles the Developmental Landscape of Arabidopsis Root.
Tian-Qi Zhang,Zhou-Geng Xu,Guan-Dong Shang,Guan-Dong Shang,Jiawei Wang,Jiawei Wang,Jiawei Wang +6 more
TL;DR: This study finds that the cells in Arabidopsis root are highly heterogeneous in their transcriptomes and identifies the expression signatures of intermediate states during root cell differentiation at single-cell resolution.
Journal ArticleDOI
Cytokinin action in response to abiotic and biotic stresses in plants.
Anne Cortleven,Jan Erik Leuendorf,Manuel Frank,Daniela Pezzetta,Sylvia Bolt,Thomas Schmülling +5 more
TL;DR: The phytohormone cytokinin was originally discovered as a regulator of cell division and later was described to be involved in regulating numerous processes in plant growth and development including meristem activity, tissue patterning, and organ size.
Journal ArticleDOI
Signaling Overview of Plant Somatic Embryogenesis.
Hugo A. Méndez-Hernández,Maharshi Ledezma-Rodríguez,Randy N. Avilez-Montalvo,Yary L. Juárez-Gómez,Analesa Skeete,Johny R. Avilez-Montalvo,Clelia De-la-Peña,Víctor M. Loyola-Vargas +7 more
TL;DR: The role of signaling is examined from the start of cell differentiation through the early steps on the embryogenic pathway, as well as its relation to a plant’s tolerance of different types of stress.
Journal ArticleDOI
Cytokinin : a developing story
Brecht Wybouw,Bert De Rybel +1 more
TL;DR: A comprehensive overview of recent findings on how cytokinin influences growth and development in plants is given, and areas for future research are highlighted.
References
More filters
Journal ArticleDOI
DNA methylation and histone modifications regulate de novo shoot regeneration in Arabidopsis by modulating WUSCHEL expression and auxin signaling.
TL;DR: It is proposed that DNA methylation and histone modifications regulate de novo shoot regeneration of Arabidopsis through modulating WUS expression and auxin signaling and demonstrates that epigenetic modifications play an evolutionarily convergent role in this process.
Journal ArticleDOI
Control of Arabidopsis apical–basal embryo polarity by antagonistic transcription factors
Zachery R. Smith,Jeff A. Long +1 more
TL;DR: It is shown that the PLT1 and PLT2 genes are direct targets of the transcriptional co-repressor TOPLESS (TPL) and thatPLT1/2 are necessary for the homeotic conversion of shoots to roots in tpl-1 mutants.
Journal ArticleDOI
Plant and animal stem cells: similar yet different
Renze Heidstra,Sabrina Sabatini +1 more
TL;DR: Components of this network have now been elucidated and provide a unique opportunity for comparing strategies that were developed in the animal and plant kingdoms, which underlie the logic of stem cell behaviour.
Journal ArticleDOI
Timing Mechanism Dependent on Cell Division Is Invoked by Polycomb Eviction in Plant Stem Cells
Bo Sun,Liang-Sheng Looi,Siyi Guo,Zemiao He,Eng-Seng Gan,Jiangbo Huang,Yifeng Xu,Wan-Yi Wee,Toshiro Ito +8 more
TL;DR: The results indicate that flower development in Arabidopsis employs cell division to provide stem cells with a window of opportunity to change fate, and the molecular circuit uncovered here may impose timing control on diverse growth and differentiation pathways in plants and animals.
Journal ArticleDOI
Concerted genomic targeting of H3K27 demethylase REF6 and chromatin-remodeling ATPase BRM in Arabidopsis
Chenlong Li,Chenlong Li,Lianfeng Gu,Lianfeng Gu,Lianfeng Gu,Lei Gao,Chen Chen,Chen Chen,Chuang-Qi Wei,Chuang-Qi Wei,Qi Qiu,Chih Wei Chien,Suikang Wang,Suikang Wang,Lihua Jiang,Lian-Feng Ai,Chia-Yang Chen,Songguang Yang,Vi Nguyen,Yanhua Qi,Michael Snyder,Alma L. Burlingame,Susanne E. Kohalmi,Shangzhi Huang,Xiaofeng Cao,Zhi-Yong Wang,Keqiang Wu,Xuemei Chen,Yuhai Cui,Yuhai Cui +29 more
TL;DR: This work identifies a new genomic targeting mechanism for an H3K27 demethylase and demonstrates its key role in recruiting the BRM chromatin remodeler.