A Two-Step Model for de novo Activation of WUSCHEL during Plant Shoot Regeneration
Tian-Qi Zhang,Tian-Qi Zhang,Heng Lian,Chuan-Miao Zhou,Lin Xu,Yuling Jiao,Jiawei Wang,Jiawei Wang +7 more
TLDR
The results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS, the homeodomain transcription factor for Arabidopsis thaliana.Abstract:
Plant cells are totipotent and competent to regenerate from differentiated organs. It has been known for six decades that cytokinin-rich medium induces shoot regeneration from callus cells. However, the underlying molecular mechanism remains elusive. The homeodomain transcription factor WUSCHEL (WUS) is essential for de novo establishment of the shoot stem cell niche in Arabidopsis thaliana We found that WUS-positive (WUS+) cells mark the shoot progenitor region during regeneration. A cytokinin-rich environment initially promotes the removal of the repressive histone mark H3K27me3 at the WUS locus in a cell cycle-dependent manner. Subsequently, the B-type ARABIDOPSIS RESPONSE REGULATORs (ARRs) ARR1, ARR2, ARR10, and ARR12, which function as transcriptional activators in the cytokinin signaling pathway, spatially activate WUS expression through binding with microRNA165/6-targeted HD-ZIP III transcription factors. Thus, our results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS.read more
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Domain-specific expression of meristematic genes is defined by the LITTLE ZIPPER protein DTM in tomato.
TL;DR: It is shown that shoot apical meristem activity in tomato is maintained by a LITTLE ZIPPER protein known as DTM, which negatively regulates a class of HD-ZIP III transcription factors, ultimately maintaining stem cell homeostasis.
Journal ArticleDOI
WIND transcription factors orchestrate wound-induced callus formation, vascular reconnection and defense response in Arabidopsis.
Akira Iwase,Yuki Kondo,Yuki Kondo,Anuphon Laohavisit,Arika Takebayashi,Momoko Ikeuchi,Keita Matsuoka,Masashi Asahina,Nobutaka Mitsuda,Ken Shirasu,Hiroo Fukuda,Keiko Sugimoto +11 more
TL;DR: The authors showed that wound stress triggers de novo organogenesis, vascular reconnection and defense response in Arabidopsis, and showed that WIND1-induced genes induce several reprogramming genes to promote callus formation at wound sites.
Journal ArticleDOI
Wound-inducible WUSCHEL RELATED HOMEOBOX 13 is required for callus growth and organ reconnection.
Momoko Ikeuchi,Akira Iwase,Tasuku Ito,Tasuku Ito,Hayato Tanaka,David S. Favero,Ayako Kawamura,Shingo Sakamoto,Mayumi Wakazaki,Toshiaki Tameshige,Toshiaki Tameshige,Haruki Fujii,Naoki Hashimoto,Takamasa Suzuki,Kazuhiro Hotta,Kiminori Toyooka,Nobutaka Mitsuda,Keiko Sugimoto +17 more
TL;DR: Wang et al. as discussed by the authors identified WUSCHEL RELATED HOMEOBOX 13 (WOX13) as a key regulator of callus formation and organ adhesion in Arabidopsis (Arabidopsis thaliana).
Journal ArticleDOI
From signals to stem cells and back again.
Denis Janocha,Jan U. Lohmann +1 more
TL;DR: The shoot apical meristem is a highly dynamic stem cell system where environmental as well as endogenous parameters are actively sensed and complex signaling pathways continuously modulate stem cell behaviour.
Journal ArticleDOI
Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs.
Martin Raspor,Václav Motyka,Abdul Rasheed Kaleri,Slavica Ninković,Ljiljana Tubić,Aleksandar Cingel,Tatjana Ćosić +6 more
TL;DR: In this paper, the authors provide an integrative interpretation of the roles for CK and auxin in the process of shoot regeneration, adding emphasis on their uptake from the regeneration media and their interaction with sucrose present in the media to their complex signaling outputs that mediate shoot regeneration.
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