A Two-Step Model for de novo Activation of WUSCHEL during Plant Shoot Regeneration
Tian-Qi Zhang,Tian-Qi Zhang,Heng Lian,Chuan-Miao Zhou,Lin Xu,Yuling Jiao,Jiawei Wang,Jiawei Wang +7 more
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TLDR
The results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS, the homeodomain transcription factor for Arabidopsis thaliana.Abstract:
Plant cells are totipotent and competent to regenerate from differentiated organs. It has been known for six decades that cytokinin-rich medium induces shoot regeneration from callus cells. However, the underlying molecular mechanism remains elusive. The homeodomain transcription factor WUSCHEL (WUS) is essential for de novo establishment of the shoot stem cell niche in Arabidopsis thaliana We found that WUS-positive (WUS+) cells mark the shoot progenitor region during regeneration. A cytokinin-rich environment initially promotes the removal of the repressive histone mark H3K27me3 at the WUS locus in a cell cycle-dependent manner. Subsequently, the B-type ARABIDOPSIS RESPONSE REGULATORs (ARRs) ARR1, ARR2, ARR10, and ARR12, which function as transcriptional activators in the cytokinin signaling pathway, spatially activate WUS expression through binding with microRNA165/6-targeted HD-ZIP III transcription factors. Thus, our results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS.read more
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The ASHR3 SET-Domain Protein is a Pivotal Upstream Coordinator for Wound-Induced Callus Formation in Arabidopsis
TL;DR: It is indicated that wounding activates a chromatin modifier, which establishes chromatin landscapes optimizing cell proliferation and reprogramming, and promotes wound-induced callus formation.
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Intermediate Developmental Phases During Regeneration
TL;DR: The intermediate developmental phases in plant regeneration are discussed and compared with the possible intermediate developmentalphase in animal regeneration and a lot more work is needed to derive a comprehensive and complete picture.
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Plant Stem Cells
A. S. Voronina,E. S. Pshennikova +1 more
TL;DR: A brief review of current data on the molecular biology of stem cells forming meristems and differentiating into various organs of angiosperms is presented.
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Auxin and cytokinin mediated regulation involved in vitro organogenesis of papaya.
Xiaobing Zhao,Jinjin Song,Qiuxia Zeng,Yaying Ma,Hanmei Fang,Liyuan Yang,Ban Deng,Juan Liu,Jingping Fang,Liping Zuo,Jingjing Yue +10 more
TL;DR: In this paper, an efficient and reproducible protocol for the induction of callus formation and shoot regeneration in Carica papaya has been proposed, where multiple factors were optimized to achieve a reproducible callus induction and regeneration.
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MET1-Dependent DNA Methylation Represses Light Signaling and Influences Plant Regeneration in Arabidopsis.
TL;DR: In this paper, the role of MET1 in de novo shoot regeneration in Arabidopsis has been investigated, showing that MET1-dependent repression of light and cytokinin signaling influences plant regeneration capacity and shoot identity establishment.
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