A Two-Step Model for de novo Activation of WUSCHEL during Plant Shoot Regeneration
Tian-Qi Zhang,Tian-Qi Zhang,Heng Lian,Chuan-Miao Zhou,Lin Xu,Yuling Jiao,Jiawei Wang,Jiawei Wang +7 more
TLDR
The results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS, the homeodomain transcription factor for Arabidopsis thaliana.Abstract:
Plant cells are totipotent and competent to regenerate from differentiated organs. It has been known for six decades that cytokinin-rich medium induces shoot regeneration from callus cells. However, the underlying molecular mechanism remains elusive. The homeodomain transcription factor WUSCHEL (WUS) is essential for de novo establishment of the shoot stem cell niche in Arabidopsis thaliana We found that WUS-positive (WUS+) cells mark the shoot progenitor region during regeneration. A cytokinin-rich environment initially promotes the removal of the repressive histone mark H3K27me3 at the WUS locus in a cell cycle-dependent manner. Subsequently, the B-type ARABIDOPSIS RESPONSE REGULATORs (ARRs) ARR1, ARR2, ARR10, and ARR12, which function as transcriptional activators in the cytokinin signaling pathway, spatially activate WUS expression through binding with microRNA165/6-targeted HD-ZIP III transcription factors. Thus, our results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS.read more
Citations
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Journal ArticleDOI
Cytokinin signaling in plant development.
TL;DR: The current understanding of cytokinin metabolism, transport and signaling is summarized, and how this phytohormone regulates changes in gene expression to mediate its pleiotropic effects is discussed.
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A Single-Cell RNA Sequencing Profiles the Developmental Landscape of Arabidopsis Root.
Tian-Qi Zhang,Zhou-Geng Xu,Guan-Dong Shang,Guan-Dong Shang,Jiawei Wang,Jiawei Wang,Jiawei Wang +6 more
TL;DR: This study finds that the cells in Arabidopsis root are highly heterogeneous in their transcriptomes and identifies the expression signatures of intermediate states during root cell differentiation at single-cell resolution.
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Cytokinin action in response to abiotic and biotic stresses in plants.
Anne Cortleven,Jan Erik Leuendorf,Manuel Frank,Daniela Pezzetta,Sylvia Bolt,Thomas Schmülling +5 more
TL;DR: The phytohormone cytokinin was originally discovered as a regulator of cell division and later was described to be involved in regulating numerous processes in plant growth and development including meristem activity, tissue patterning, and organ size.
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Signaling Overview of Plant Somatic Embryogenesis.
Hugo A. Méndez-Hernández,Maharshi Ledezma-Rodríguez,Randy N. Avilez-Montalvo,Yary L. Juárez-Gómez,Analesa Skeete,Johny R. Avilez-Montalvo,Clelia De-la-Peña,Víctor M. Loyola-Vargas +7 more
TL;DR: The role of signaling is examined from the start of cell differentiation through the early steps on the embryogenic pathway, as well as its relation to a plant’s tolerance of different types of stress.
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Cytokinin : a developing story
Brecht Wybouw,Bert De Rybel +1 more
TL;DR: A comprehensive overview of recent findings on how cytokinin influences growth and development in plants is given, and areas for future research are highlighted.
References
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Journal ArticleDOI
A mechanistic framework for noncell autonomous stem cell induction in Arabidopsis
TL;DR: Evidence is provided that the stem cell inducing transcription factor WUSCHEL (WUS), expressed in the niche, moves to the stem cells via plasmodesmata in a highly regulated fashion and that this movement is required for WUS function and, thus, stem cell activity in Arabidopsis thaliana.
Journal ArticleDOI
Three Type-B Response Regulators, ARR1, ARR10, and ARR12 Play Essential but Redundant Roles in Cytokinin Signal Transduction Throughout the Life Cycle of Arabidopsis thaliana
TL;DR: It is shown that the observed cytokinin-associated phenotypes of arr1 arr10 arr12 were very severe and highly analogous to those observed for certain ahk2 ahk3 ahk4/cre1 triple mutants, which have virtually no cytokinIn receptor to propagate the phosphorelay signal transduction network.
Journal ArticleDOI
AGAMOUS Terminates Floral Stem Cell Maintenance in Arabidopsis by Directly Repressing WUSCHEL through Recruitment of Polycomb Group Proteins
Xigang Liu,Yun Ju Kim,Ralf Müller,Rae Eden Yumul,Chunyan Liu,Yanyun Pan,Xiaofeng Cao,Justin Goodrich,Xuemei Chen +8 more
TL;DR: It is shown that AG directly represses WUS expression by binding to the WUS locus and recruiting, directly or indirectly, PcG that methylates histone H3 Lys-27 at WUS.
Journal ArticleDOI
Pattern of Auxin and Cytokinin Responses for Shoot Meristem Induction Results from the Regulation of Cytokinin Biosynthesis by AUXIN RESPONSE FACTOR3
Zhi Juan Cheng,Liang Wang,Wei Sun,Yan Zhang,Chao Zhou,Ying Hua Su,Wei Li,Tian Tian Sun,Xiang Yu Zhao,Xing Guo Li,Youfa Cheng,Yunde Zhao,Qi Xie,Xian Sheng Zhang +13 more
TL;DR: It is shown that spatiotemporal biosynthesis and polar transport resulted in local auxin distribution in Arabidopsis, which in turn determined the cytokinin response during de novo shoot regeneration, and it was shown that ARF3 directly bound to the promoter of AtIPT5 and negatively regulated AtipT5 expression.
Journal ArticleDOI
Epigenetic reprogramming that prevents transgenerational inheritance of the vernalized state
Pedro Crevillén,Hongchun Yang,Xia Cui,Christiaan Greeff,Martin Trick,Qi Qiu,Xiaofeng Cao,Caroline Dean +7 more
TL;DR: It is shown that a hypomorphic mutation in the jumonji-domain-containing protein ELF6 impaired the reactivation of FLC in reproductive tissues, leading to the inheritance of a partially vernalized state.