A Two-Step Model for de novo Activation of WUSCHEL during Plant Shoot Regeneration
Tian-Qi Zhang,Tian-Qi Zhang,Heng Lian,Chuan-Miao Zhou,Lin Xu,Yuling Jiao,Jiawei Wang,Jiawei Wang +7 more
TLDR
The results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS, the homeodomain transcription factor for Arabidopsis thaliana.Abstract:
Plant cells are totipotent and competent to regenerate from differentiated organs. It has been known for six decades that cytokinin-rich medium induces shoot regeneration from callus cells. However, the underlying molecular mechanism remains elusive. The homeodomain transcription factor WUSCHEL (WUS) is essential for de novo establishment of the shoot stem cell niche in Arabidopsis thaliana We found that WUS-positive (WUS+) cells mark the shoot progenitor region during regeneration. A cytokinin-rich environment initially promotes the removal of the repressive histone mark H3K27me3 at the WUS locus in a cell cycle-dependent manner. Subsequently, the B-type ARABIDOPSIS RESPONSE REGULATORs (ARRs) ARR1, ARR2, ARR10, and ARR12, which function as transcriptional activators in the cytokinin signaling pathway, spatially activate WUS expression through binding with microRNA165/6-targeted HD-ZIP III transcription factors. Thus, our results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS.read more
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Arabidopsis ATXR2 represses de novo shoot organogenesis in the transition from callus to shoot formation.
Kyoung-Hee Lee,Ok-Sun Park,Ji Yun Go,Jihyeon Yu,Jun Hee Han,Jungmook Kim,Sangsu Bae,Yu Jin Jung,Pil Joon Seo +8 more
TL;DR: In this paper, the ATXR2/ARR1-type-A ARR module transiently represses cytokinin signaling and thereby de novo shoot regeneration, and the atxr2-1 mutant calli exhibit enhanced shoot regeneration with low expression of ARR5 and ARR7.
Journal ArticleDOI
ARR22 overexpression can suppress plant Two-Component Regulatory Systems
Niklas Wallmeroth,Daniel Jeschke,Daniel Slane,Janine Nägele,Manikandan Veerabagu,Virtudes Mira-Rodado,Kenneth W. Berendzen +6 more
TL;DR: The approach can be used to investigate gene signaling networks involving the TCS by leveraging ARR22 as a TCS inhibitor along with B-type ARR overexpression and mimicked effects previously observed only in transgenic plants for all of the T CS proteins studied.
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Molecular and genetic pathways for optimizing spikelet development and grain yield
TL;DR: This review focuses on recent progress on spikelet initiation, and spikelet and floret fertility, by comparing results from Arabidopsis with that of rice, sorghum, maize, barley, wheat, Brachypodium distachyon, and Setaria viridis.
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Rejuvenation increases leaf biomass and flavonoid accumulation in Ginkgo biloba
Zhaogeng Lu,Likui Zhu,Jinkai Lu,Nan Shen,Lu-Ning Wang,Sian Liu,Qingjie Wang,Wanwen Yu,Hisashi Kato-Noguchi,Weixing Li,Biao Jin,Liang Wang,Jinxing Lin +12 more
TL;DR: It is shown that truncation can stimulate tree rejuvenation by altering hormone levels, representing an effective and feasible approach for enhancing the biomass and flavonoid content of G. biloba leaves.
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Transcriptional activation of auxin biosynthesis drives developmental reprogramming of differentiated cells
TL;DR: In this paper , the authors demonstrate that transcriptional activation of auxin biosynthesis is crucial for reprogramming differentiated Arabidopsis (Arabidopsis thaliana) leaf cells.
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