A Two-Step Model for de novo Activation of WUSCHEL during Plant Shoot Regeneration
Tian-Qi Zhang,Tian-Qi Zhang,Heng Lian,Chuan-Miao Zhou,Lin Xu,Yuling Jiao,Jiawei Wang,Jiawei Wang +7 more
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TLDR
The results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS, the homeodomain transcription factor for Arabidopsis thaliana.Abstract:
Plant cells are totipotent and competent to regenerate from differentiated organs. It has been known for six decades that cytokinin-rich medium induces shoot regeneration from callus cells. However, the underlying molecular mechanism remains elusive. The homeodomain transcription factor WUSCHEL (WUS) is essential for de novo establishment of the shoot stem cell niche in Arabidopsis thaliana We found that WUS-positive (WUS+) cells mark the shoot progenitor region during regeneration. A cytokinin-rich environment initially promotes the removal of the repressive histone mark H3K27me3 at the WUS locus in a cell cycle-dependent manner. Subsequently, the B-type ARABIDOPSIS RESPONSE REGULATORs (ARRs) ARR1, ARR2, ARR10, and ARR12, which function as transcriptional activators in the cytokinin signaling pathway, spatially activate WUS expression through binding with microRNA165/6-targeted HD-ZIP III transcription factors. Thus, our results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS.read more
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The WUSCHEL-related homeobox transcription factor MtWOX9-1 stimulates somatic embryogenesis in Medicago truncatula
V. E. Tvorogova,Yulia A. Fedorova,Yulia A. Fedorova,Elina A. Potsenkovskaya,Andrei A. Kudriashov,Elena P. Efremova,Valeria A. Kvitkovskaya,Tezera W. Wolabu,Fei Zhang,Fei Zhang,Million Tadege,Ludmila A Lutova +11 more
TL;DR: The data obtained suggest that stimulation of MtWOX9-1 expression may have positive impact on plant biotechnology in improving the transformation and regeneration capacity of recalcitrant plants.
Journal ArticleDOI
Advances in Plant Regeneration: Shake, Rattle and Roll
TL;DR: This review will provide additional insights into the physiological and molecular framework of plant regeneration, including both direct and indirect de novo organ formation and somatic embryogenesis, and the key role of intrinsic and extrinsic constraints for cell reprogramming during plant regeneration is discussed.
Journal ArticleDOI
The Type-B Cytokinin Response Regulator ARR1 Inhibits Shoot Regeneration in an ARR12-Dependent Manner in Arabidopsis.
TL;DR: It is shown that ARR 12 is a central enhancer of callus formation and shoot regeneration, whereas ARR1 is a strong inhibitor of this process that counteracts the positive effect of ARR12, and novel molecular pathways linking cytokinin signaling, the CLV3 regulator, and auxin signaling are revealed.
Journal ArticleDOI
The explant developmental stage profoundly impacts small RNA-mediated regulation at the dedifferentiation step of maize somatic embryogenesis
Vasti T. Juárez-González,Brenda A. López-Ruiz,Patricia Baldrich,Eduardo Luján-Soto,Blake C. Meyers,Blake C. Meyers,Tzvetanka D. Dinkova +6 more
TL;DR: It is concluded that sRNA-target regulation operating within the 15 DAP immature embryo explant provides key molecular hints as to why this stage is relevant for callus induction with successful proliferation and plant regeneration.
Journal ArticleDOI
MicroRNA166 Monitors SPOROCYTELESS/NOZZLE for Building of the Anther Internal Boundary.
TL;DR: It is reported that microRNA166 (miR166) and its target PHABULOSA (PHB) regulate SPOROCYTELESS/NOZZLE (SPL/NZZ), which controls microsporogenesis, and link adaxial/abaxial polarity to microsporaogenesis in building of the internal boundary of anthers and advance the concepts underlying the establishment of theinternal structure of male organs.
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