A Two-Step Model for de novo Activation of WUSCHEL during Plant Shoot Regeneration
Tian-Qi Zhang,Tian-Qi Zhang,Heng Lian,Chuan-Miao Zhou,Lin Xu,Yuling Jiao,Jiawei Wang,Jiawei Wang +7 more
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TLDR
The results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS, the homeodomain transcription factor for Arabidopsis thaliana.Abstract:
Plant cells are totipotent and competent to regenerate from differentiated organs. It has been known for six decades that cytokinin-rich medium induces shoot regeneration from callus cells. However, the underlying molecular mechanism remains elusive. The homeodomain transcription factor WUSCHEL (WUS) is essential for de novo establishment of the shoot stem cell niche in Arabidopsis thaliana We found that WUS-positive (WUS+) cells mark the shoot progenitor region during regeneration. A cytokinin-rich environment initially promotes the removal of the repressive histone mark H3K27me3 at the WUS locus in a cell cycle-dependent manner. Subsequently, the B-type ARABIDOPSIS RESPONSE REGULATORs (ARRs) ARR1, ARR2, ARR10, and ARR12, which function as transcriptional activators in the cytokinin signaling pathway, spatially activate WUS expression through binding with microRNA165/6-targeted HD-ZIP III transcription factors. Thus, our results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS.read more
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A B-ARR-mediated cytokinin transcriptional network directs hormone cross-regulation and shoot development
Mingtang Xie,Hongyu Chen,Ling Huang,Ryan C. O’Neil,Ryan C. O’Neil,Maxim N. Shokhirev,Joseph R. Ecker +6 more
TL;DR: It is found that WUSCHEL, a key gene required for apical meristem maintenance, is a cytokinin-dependent B-ARR target gene, demonstrating the importance of the cytokinIn transcription factor network in shoot development.
Journal ArticleDOI
A single-cell analysis of the Arabidopsis vegetative shoot apex.
TL;DR: In this article, the authors used single-cell RNA sequencing to define the cellular taxonomy of the Arabidopsis vegetative shoot apex at the transcriptome level, and found that the shoot apex is composed of highly heterogeneous cells, which can be partitioned into 7 broad populations with 23 transcriptionally distinct cell clusters.
Journal ArticleDOI
Root stem cell niche organizer specification by molecular convergence of PLETHORA and SCARECROW transcription factor modules
Akie Shimotohno,Renze Heidstra,Renze Heidstra,Ikram Blilou,Ikram Blilou,Ben Scheres,Ben Scheres +6 more
TL;DR: It is shown that PLT and SCR genetically and physically interact with plant-specific teosinte-branched cycloidea PCNA (TCP) transcription factors to specify the stem cell niche during embryogenesis and maintain organizer cells post-embryonically.
Journal ArticleDOI
AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration.
Bin-Bin Ye,Guan-Dong Shang,Yu Pan,Zhou-Geng Xu,Chuan-Miao Zhou,Ying-Bo Mao,Ning Bao,Lijun Sun,Tongda Xu,Jiawei Wang,Jiawei Wang +10 more
TL;DR: It is shown that the increased levels of three closely-related miR156-targeted Arabidopsis (Arabidopsis thaliana) SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors, SPL2, SPL10, and SPL11, suppress root regeneration with age by inhibiting wound-induced auxin biosynthesis.
Journal ArticleDOI
The WOX11-LBD16 Pathway Promotes Pluripotency Acquisition in Callus Cells During De Novo Shoot Regeneration in Tissue Culture.
TL;DR: It is shown that the Arabidopsis thaliana transcription factor gene LATERAL ORGAN BOUNDARIES DOMAIN16 (LBD16) is involved in pluripotency acquisition in callus cells, and that LBD16 may function in the establishment of the root primordium-like identity in the newly formed callus.
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