A Two-Step Model for de novo Activation of WUSCHEL during Plant Shoot Regeneration
Tian-Qi Zhang,Tian-Qi Zhang,Heng Lian,Chuan-Miao Zhou,Lin Xu,Yuling Jiao,Jiawei Wang,Jiawei Wang +7 more
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TLDR
The results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS, the homeodomain transcription factor for Arabidopsis thaliana.Abstract:
Plant cells are totipotent and competent to regenerate from differentiated organs. It has been known for six decades that cytokinin-rich medium induces shoot regeneration from callus cells. However, the underlying molecular mechanism remains elusive. The homeodomain transcription factor WUSCHEL (WUS) is essential for de novo establishment of the shoot stem cell niche in Arabidopsis thaliana We found that WUS-positive (WUS+) cells mark the shoot progenitor region during regeneration. A cytokinin-rich environment initially promotes the removal of the repressive histone mark H3K27me3 at the WUS locus in a cell cycle-dependent manner. Subsequently, the B-type ARABIDOPSIS RESPONSE REGULATORs (ARRs) ARR1, ARR2, ARR10, and ARR12, which function as transcriptional activators in the cytokinin signaling pathway, spatially activate WUS expression through binding with microRNA165/6-targeted HD-ZIP III transcription factors. Thus, our results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS.read more
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The ancient wave of polyploidization events in flowering plants and their facilitated adaptation to environmental stress.
Liangsheng Zhang,Sheng-Dan Wu,Xiaojun Chang,Xiuyun Wang,Yunpeng Zhao,Yiping Xia,Robert N. Trigiano,Yuannian Jiao,Fei Chen +8 more
TL;DR: This ancient wave of paleopolyploidy events could have significantly contributed to the adaptation of angiosperms to environmental changes, and potentially promoted the wide diversification of flowering plants.
Journal ArticleDOI
Primed histone demethylation regulates shoot regenerative competency.
Hiroya Ishihara,Kaoru Sugimoto,Paul T. Tarr,Haruka Temman,Satoshi Kadokura,Yayoi Inui,Takuya Sakamoto,Taku Sasaki,Mitsuhiro Aida,Takamasa Suzuki,Soichi Inagaki,Kengo Morohashi,Motoaki Seki,Tetsuji Kakutani,Tetsuji Kakutani,Tetsuji Kakutani,Elliot M. Meyerowitz,Sachihiro Matsunaga +17 more
TL;DR: It is shown that acquisition of regenerative capacity of callus-forming cells requires a lysine-specific demethylase that removes H3K4me2 to prime gene expression in response to regenerative cues.
Journal ArticleDOI
De novo shoot organogenesis during plant regeneration.
TL;DR: This study reconstitute the shoot regeneration process with four phases, including pluripotency acquisition, shoot promeristem formation, establishment of confined shoot progenitor, and shoot outgrowth, and highlights recent advances in the molecular and cellular events involved in DNSO and regulatory mechanisms behind key steps of DNSO.
Journal ArticleDOI
Thioredoxin-Mediated ROS Homeostasis Explains Natural Variation in Plant Regeneration.
Hui Zhang,Ting Ting Zhang,Hui Liu,De Ying Shi,Meng Wang,Xiao Min Bie,Xing Guo Li,Xian Sheng Zhang +7 more
TL;DR: This study uncovers a novel regulatory mechanism by which thioredoxin-dependent redox modification regulates de novo shoot initiation via the modulation of ROS homeostasis and provides new insights into improving the capacity of plant regeneration.
Journal ArticleDOI
Transcriptional circuits in control of shoot stem cell homeostasis
TL;DR: Recent findings mainly from studies in Arabidopsis are highlighted, and research progress on understanding how multiple transcriptional circuits integrate and function at different cell layers to control the WUS-CLV3 loop and stem cell homeostasis is summarized.
References
More filters
Journal ArticleDOI
Clustal W and Clustal X version 2.0
Mark A. Larkin,Gordon Blackshields,Nigel P. Brown,R. Chenna,Paul A. McGettigan,Hamish McWilliam,Franck Valentin,Iain M. Wallace,Andreas Wilm,Rodrigo Lopez,J.D. Thompson,Toby J. Gibson,Desmond G. Higgins +12 more
TL;DR: The Clustal W and ClUSTal X multiple sequence alignment programs have been completely rewritten in C++ to facilitate the further development of the alignment algorithms in the future and has allowed proper porting of the programs to the latest versions of Linux, Macintosh and Windows operating systems.
Journal ArticleDOI
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
Steven J. Clough,Andrew F. Bent +1 more
TL;DR: The modified method should facilitate high-throughput transformation of Arabidopsis for efforts such as T-DNA gene tagging, positional cloning, or attempts at targeted gene replacement.
Journal ArticleDOI
Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis.
TL;DR: The transient gene expression system using Arabidopsis mesophyll protoplasts has proven an important and versatile tool for conducting cell-based experiments using molecular, cellular, biochemical, genetic, genomic and proteomic approaches to analyze the functions of diverse signaling pathways and cellular machineries.
Journal Article
Chemical regulation of growth and organ formation in plant tissues cultured in vitro.
F Skoog,C O Miller +1 more
Journal ArticleDOI
The Stem Cell Population of Arabidopsis Shoot Meristems Is Maintained by a Regulatory Loop between the CLAVATA and WUSCHEL Genes
TL;DR: This paper showed that the shoot meristem has properties of a self-regulatory system in which WUS/CLV interactions establish a feedback loop between the stem cells and the underlying organizing center.