A Two-Step Model for de novo Activation of WUSCHEL during Plant Shoot Regeneration
Tian-Qi Zhang,Tian-Qi Zhang,Heng Lian,Chuan-Miao Zhou,Lin Xu,Yuling Jiao,Jiawei Wang,Jiawei Wang +7 more
TLDR
The results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS, the homeodomain transcription factor for Arabidopsis thaliana.Abstract:
Plant cells are totipotent and competent to regenerate from differentiated organs. It has been known for six decades that cytokinin-rich medium induces shoot regeneration from callus cells. However, the underlying molecular mechanism remains elusive. The homeodomain transcription factor WUSCHEL (WUS) is essential for de novo establishment of the shoot stem cell niche in Arabidopsis thaliana We found that WUS-positive (WUS+) cells mark the shoot progenitor region during regeneration. A cytokinin-rich environment initially promotes the removal of the repressive histone mark H3K27me3 at the WUS locus in a cell cycle-dependent manner. Subsequently, the B-type ARABIDOPSIS RESPONSE REGULATORs (ARRs) ARR1, ARR2, ARR10, and ARR12, which function as transcriptional activators in the cytokinin signaling pathway, spatially activate WUS expression through binding with microRNA165/6-targeted HD-ZIP III transcription factors. Thus, our results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS.read more
Citations
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Journal ArticleDOI
Cytokinin signaling in plant development.
TL;DR: The current understanding of cytokinin metabolism, transport and signaling is summarized, and how this phytohormone regulates changes in gene expression to mediate its pleiotropic effects is discussed.
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A Single-Cell RNA Sequencing Profiles the Developmental Landscape of Arabidopsis Root.
Tian-Qi Zhang,Zhou-Geng Xu,Guan-Dong Shang,Guan-Dong Shang,Jiawei Wang,Jiawei Wang,Jiawei Wang +6 more
TL;DR: This study finds that the cells in Arabidopsis root are highly heterogeneous in their transcriptomes and identifies the expression signatures of intermediate states during root cell differentiation at single-cell resolution.
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Cytokinin action in response to abiotic and biotic stresses in plants.
Anne Cortleven,Jan Erik Leuendorf,Manuel Frank,Daniela Pezzetta,Sylvia Bolt,Thomas Schmülling +5 more
TL;DR: The phytohormone cytokinin was originally discovered as a regulator of cell division and later was described to be involved in regulating numerous processes in plant growth and development including meristem activity, tissue patterning, and organ size.
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Signaling Overview of Plant Somatic Embryogenesis.
Hugo A. Méndez-Hernández,Maharshi Ledezma-Rodríguez,Randy N. Avilez-Montalvo,Yary L. Juárez-Gómez,Analesa Skeete,Johny R. Avilez-Montalvo,Clelia De-la-Peña,Víctor M. Loyola-Vargas +7 more
TL;DR: The role of signaling is examined from the start of cell differentiation through the early steps on the embryogenic pathway, as well as its relation to a plant’s tolerance of different types of stress.
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Cytokinin : a developing story
Brecht Wybouw,Bert De Rybel +1 more
TL;DR: A comprehensive overview of recent findings on how cytokinin influences growth and development in plants is given, and areas for future research are highlighted.
References
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Journal ArticleDOI
Regulation of WUSCHEL Transcription in the Stem Cell Niche of the Arabidopsis Shoot Meristem
Isabel Bäurle,Thomas Laux +1 more
TL;DR: The results indicate that the diverse regulatory pathways that control the stem cells in the shoot meristem converge at these two short sequence elements of the WUS promoter, suggesting that the integration of regulatory signals takes place at the level of a central transactivating complex.
Journal ArticleDOI
A Regulatory Framework for Shoot Stem Cell Control Integrating Metabolic, Transcriptional, and Phytohormone Signals
Christoph Schuster,Christophe Gaillochet,Anna Medzihradszky,Wolfgang Busch,Gabor Daum,Melanie Krebs,Andreas Kehle,Jan U. Lohmann +7 more
TL;DR: It is demonstrated that the bHLH transcription factor HECATE1 (HEC1) is a target of WUS and that it contributes to SAM function by promoting stem cell proliferation, while antagonizing niche cell activity.
Journal ArticleDOI
The Transcriptional Repressor ARR1-SRDX Suppresses Pleiotropic Cytokinin Activities in Arabidopsis
Alexander Heyl,Eswar Ramireddy,Wolfram G. Brenner,Michael Riefler,Joke Allemeersch,Thomas Schmülling +5 more
TL;DR: The suppression of pleiotropic cytokinin activities by a dominant repressor version of a B-type ARR indicates that this protein family is involved in mediating most, if not all, of the cytokinIn activities in Arabidopsis.
Book ChapterDOI
Shoot apical meristem form and function.
TL;DR: This work has shown that advances in molecular and computational techniques are becoming integrated with data from traditional molecular genetics methodologies to develop a framework for understanding the central principles of SAM function.
Journal ArticleDOI
Two-Step Regulation of a Meristematic Cell Population Acting in Shoot Branching in Arabidopsis
Bihai Shi,Cui Zhang,Caihuan Tian,Jin Wang,Quan Wang,Tengfei Xu,Tengfei Xu,Yan Xu,Carolyn Ohno,Robert Sablowski,Marcus G. Heisler,Klaus Theres,Ying Wang,Yuling Jiao +13 more
TL;DR: Using live-cell imaging of leaf axil cells, it is shown that the initiation of axillary meristems requires a meristematic cell population continuously expressing the meristsem marker SHOOT MERISTEMLESS (STM), and this suggests that some cells undergo irreversible commitment to a developmental fate.