A Two-Step Model for de novo Activation of WUSCHEL during Plant Shoot Regeneration
Tian-Qi Zhang,Tian-Qi Zhang,Heng Lian,Chuan-Miao Zhou,Lin Xu,Yuling Jiao,Jiawei Wang,Jiawei Wang +7 more
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TLDR
The results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS, the homeodomain transcription factor for Arabidopsis thaliana.Abstract:
Plant cells are totipotent and competent to regenerate from differentiated organs. It has been known for six decades that cytokinin-rich medium induces shoot regeneration from callus cells. However, the underlying molecular mechanism remains elusive. The homeodomain transcription factor WUSCHEL (WUS) is essential for de novo establishment of the shoot stem cell niche in Arabidopsis thaliana We found that WUS-positive (WUS+) cells mark the shoot progenitor region during regeneration. A cytokinin-rich environment initially promotes the removal of the repressive histone mark H3K27me3 at the WUS locus in a cell cycle-dependent manner. Subsequently, the B-type ARABIDOPSIS RESPONSE REGULATORs (ARRs) ARR1, ARR2, ARR10, and ARR12, which function as transcriptional activators in the cytokinin signaling pathway, spatially activate WUS expression through binding with microRNA165/6-targeted HD-ZIP III transcription factors. Thus, our results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS.read more
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Journal ArticleDOI
Cytokinin signaling in plant development.
TL;DR: The current understanding of cytokinin metabolism, transport and signaling is summarized, and how this phytohormone regulates changes in gene expression to mediate its pleiotropic effects is discussed.
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A Single-Cell RNA Sequencing Profiles the Developmental Landscape of Arabidopsis Root.
Tian-Qi Zhang,Zhou-Geng Xu,Guan-Dong Shang,Guan-Dong Shang,Jiawei Wang,Jiawei Wang,Jiawei Wang +6 more
TL;DR: This study finds that the cells in Arabidopsis root are highly heterogeneous in their transcriptomes and identifies the expression signatures of intermediate states during root cell differentiation at single-cell resolution.
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Cytokinin action in response to abiotic and biotic stresses in plants.
Anne Cortleven,Jan Erik Leuendorf,Manuel Frank,Daniela Pezzetta,Sylvia Bolt,Thomas Schmülling +5 more
TL;DR: The phytohormone cytokinin was originally discovered as a regulator of cell division and later was described to be involved in regulating numerous processes in plant growth and development including meristem activity, tissue patterning, and organ size.
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Signaling Overview of Plant Somatic Embryogenesis.
Hugo A. Méndez-Hernández,Maharshi Ledezma-Rodríguez,Randy N. Avilez-Montalvo,Yary L. Juárez-Gómez,Analesa Skeete,Johny R. Avilez-Montalvo,Clelia De-la-Peña,Víctor M. Loyola-Vargas +7 more
TL;DR: The role of signaling is examined from the start of cell differentiation through the early steps on the embryogenic pathway, as well as its relation to a plant’s tolerance of different types of stress.
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TL;DR: A comprehensive overview of recent findings on how cytokinin influences growth and development in plants is given, and areas for future research are highlighted.
References
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Journal ArticleDOI
WUSCHEL protein movement mediates stem cell homeostasis in the Arabidopsis shoot apex
Ram Kishor Yadav,Mariano Perales,Jérémy Gruel,Thomas Girke,Henrik Jönsson,G. Venugopala Reddy +5 more
TL;DR: It is shown that maintenance of the WUS gradient is essential to regulate stem cell number and Migration of a stem cell-inducing transcription factor into adjacent cells to activate a negative regulator, thereby restricting its own accumulation, is a theme that is unique to plant stem cell niches.
Journal ArticleDOI
Multiple feedback loops through cytokinin signaling control stem cell number within the Arabidopsis shoot meristem
TL;DR: It is found that localized perception of the plant hormone cytokinin establishes a spatial domain in which cell fate is respecified through induction of the master regulator WUSCHEL as cells are displaced during growth.
Journal ArticleDOI
Multiple Type-B Response Regulators Mediate Cytokinin Signal Transduction in Arabidopsis
Michael G. Mason,Dennis E. Mathews,D. Aaron Argyros,Bridey B. Maxwell,Joseph J. Kieber,Jose M. Alonso,Joseph R. Ecker,G. Eric Schaller +7 more
TL;DR: Results indicate that there is functional overlap among the type-B ARRs and that they act as positive regulators of cytokinin signal transduction.
Journal ArticleDOI
REVOLUTA regulates meristem initiation at lateral positions
TL;DR: The REV gene is identified and found it encodes a predicted homeodomain/leucine zipper transcription factor that also contains a START sterol-lipid binding domain, and it is proposed that REV acts at lateral positions to activate the expression of known meristem regulators.
Journal ArticleDOI
Slicing across Kingdoms: Regeneration in Plants and Animals
TL;DR: The question remains in both plants and animals whether regeneration invokes embryogenesis, generic patterning mechanisms, or unique circuitry comprised of well-established patterning genes.