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Open AccessJournal ArticleDOI

Catalytic hairpin DNA assembly-based chemiluminescent assay for the detection of short SARS-CoV-2 target cDNA

Ji Yoon Do, +2 more
- 01 Oct 2021 - 
- Vol. 233, pp 122505-122505
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TLDR
In this article, the catalytic hairpin DNA assembly (CHA) approach was combined with enzyme-linked immunosorbent assay (ELISA)-mimicking techniques for a simple, sensitive, and sequence-specific colorimetric assay to detect short SARS CoV-2 target cDNA.
About
This article is published in Talanta.The article was published on 2021-10-01 and is currently open access. It has received 15 citations till now. The article focuses on the topics: Complementary DNA.

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Citations
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Journal ArticleDOI

Electrochemical sensors for the detection of SARS-CoV-2 virus

TL;DR: In this paper, a review of the recently developed electrochemical sensors that are focused on the detection of viral nucleic acid, immunoglobulin, antigen, and the entire viral particles is presented.
Journal ArticleDOI

Electrochemical sensors for the detection of SARS-CoV-2 virus

TL;DR: In this article , a review of the recently developed electrochemical sensors that are focused on the detection of viral nucleic acid, immunoglobulin, antigen, and the entire viral particles is presented.
Journal ArticleDOI

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective.

TL;DR: A comprehensive review of existing DNAzyme-based sensors can be found in this paper, with a focus on their respective advantages, drawbacks, and optimal conditions for use, as well as emerging and future trends in the development of DNA-based biosensors are discussed.
Journal ArticleDOI

A Study of the Detection of SARS-CoV-2 ORF1ab Gene by the Use of Electrochemiluminescent Biosensor Based on Dual-Probe Hybridization

TL;DR: A new method was created for detecting SARS-CoV-2 of the open reading frames 1ab (ORF1ab) target gene by a electrochemiluminescence (ECL) biosensor based on dual-probe hybridization through the use of a detection model of “magnetic capture probes—targeted nucleic acids—Ru(bpy)32+ labeled signal probes”.
References
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Journal ArticleDOI

Triggered amplification by hybridization chain reaction.

TL;DR: This work introduces the concept of hybridization chain reaction (HCR), in which stable DNA monomers assemble only upon exposure to a target DNA fragment, which allows DNA to act as an amplifying transducer for biosensing applications.
Journal ArticleDOI

Programming biomolecular self-assembly pathways

TL;DR: Diverse molecular self-assembly and disassembly pathways are program using a ‘reaction graph’ abstraction to specify complementarity relationships between modular domains in a versatile DNA hairpin motif.
Journal ArticleDOI

Rapid and quantitative detection of human adenovirus DNA by real-time PCR.

TL;DR: Real‐time PCR with consensus primer and probe sequences for a conserved region of the hexon gene is designed and evaluated and is found to be a sensitive and quantitative procedure for the detection of adenovirus infections.
Journal ArticleDOI

Rational, modular adaptation of enzyme-free DNA circuits to multiple detection methods

TL;DR: This work designed and characterized a simplified DNA circuit based on catalyzed hairpin assembly, and applied it to multiple different analytical formats, including fluorescent, colorimetric, and electrochemical and signaling, demonstrating that catalyzedhairpin assembly is suitable for analyte detection and signal amplification in a ‘plug-and-play’ fashion.
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