Journal ArticleDOI
Characteristics of Perforin Expressing Lymphocytes Within the First Trimester Decidua of Human Pregnancy
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TLDR
The aim was to investigate the distribution and the phenotype of P+ cells in decidua of pregnancy and to find out if the number of perforin‐positive cells in pregnancy is larger than in any other pathological condition.Abstract:
PROBLEM: The number of perforin (P)-positive cells in decidua of pregnancy is larger than that observed in any other pathological condition. The aim was to investigate the distribution and the phenotype of P + cells. METHOD: Decidual tissue was obtained from the first trimester vaginal termination of pregnancy. Tissue distribution of P + cells was analyzed by immunohistochemistry. The method for simultaneous measurement of P and cell surface is presented. RESULTS: There is no difference in number and distribution of P + cells between decidua basalis (DB) and decidua parietalis (DP). The percentage of P + decidual lymphocytes (DL) is two times higher than in peripheral blood lymphocytes (PBL) (55% vs. 27%), and the prevalent phenotype is CD3 - CD4 - CD8 - CD2 + (95%) CD11c + (68%) and CD56 + (82%). CD56 bright+ DL are also P bright+ and this is the largest DL subpopulation (42.4% DL). Two different subpopulations of CD8 + DL exist: 1) CD8 bright+ , which are CD3 + CD56 - P - and 2) CD8 dim+ , which are CD3 - CD56 + P + . CONCLUSION: P expressing DL are prevalently nonclassical NK cells (CD16 - ) with low cytolytic activity but fully equipped with potent cytolytic machinery (P bright+ ). There are no classical cytotoxic lymphocytes (CTL) (CD3 + CD8 + P + ) in the decidua, and all CD8 + P + cells are CD3 - CD56 + . The number of P + cells is even higher in DP in the vicinity of noninvasvie trophoblast, than in DBread more
Citations
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Journal ArticleDOI
Progesterone and the immunology of pregnancy
TL;DR: The foetal-placental unit is a semi-allograft and the immunological recognition of pregnancy, together with the subsequent response of the maternal immune system, is necessary for a successful pregnancy.
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Transforming growth factor β—a mediator of immune deviation in seminal plasma
TL;DR: A better understanding of the significance and role of TGFβ in semen will facilitate development of novel therapies for immune-based infertility disorders and explain epidemiological observations linking acute and cumulative exposure to semen with successful placental development and pregnancy outcome.
Journal ArticleDOI
The decidua—the maternal bed embracing the embryo—maintains the pregnancy
TL;DR: The structure of the decidua is reviewed, which is composed of terminally differentiated uterine stromal cells, blood vessels, and a number of repertoire of uterine local immune cells, including the well-known uterine natural killer cells and recently discovered innate lymphoid cells (ILCs).
Journal ArticleDOI
Age-Related Decline of Perforin Expression in Human Cytotoxic T Lymphocytes and Natural Killer Cells
Daniel Rukavina,Gordana Laškarin,Gordana Rubeša,Natasa Strbo,Ivica Bedenicki,Darko Manestar,Mario Glavas,Stephen E. Christmas,Eckhard R. Podack +8 more
TL;DR: Functional tests clearly showed the deficiency in early spontaneous cytotoxic potential of PBL from elderly persons due to relative P deficiency, which can be corrected by stimulation of cytolytic cells with target cells and interleukin-2 (IL-2).
Journal ArticleDOI
Progesterone-induced blocking factor inhibits degranulation of natural killer cells.
TL;DR: Peripheral blood lymphocytes of healthy pregnant women produce progesterone‐induced blocking factor (PIBF), which inhibits NK activity, which might contribute to low decidual NK activity during the first trimester of pregnancy.
References
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Journal ArticleDOI
A class I antigen, HLA-G, expressed in human trophoblasts.
Susan Kovats,Elliott K. Main,Clifford Librach,Marcia Stubblebine,Susan J. Fisher,Robert DeMars +5 more
TL;DR: HLA-G is subject to both cell type-specific and developmental regulation and is expressed in early gestation human cytotrophoblasts and is present in choriocarcinoma cell lines studied.
Journal Article
Comparative studies of human FcRIII-positive and negative natural killer cells.
TL;DR: Three subpopulations of peripheral blood NK cells based on the surface expression of CD56 and CD16 are identified and proposed that these NK cell subsets represent portions of the NK cell differentiation pathway present in the peripheral blood.
Journal ArticleDOI
Structure and function of human perforin.
Mathias G. Lichtenheld,Kristin J. Olsen,Ping Lu,David M. Lowrey,Arif Hameed,Hans Hengartner,Eckhard R. Podack +6 more
TL;DR: The primary structure of human P1 is determined, its functional properties are re-examine, and the question of homologous restriction factor is addressed.
Journal ArticleDOI
Immunocytochemical characterization of the unusual large granular lymphocytes in human endometrium throughout the menstrual cycle.
TL;DR: The findings suggest that the recruitment of these large granular lymphocytes to the uterus is under hormonal control and is not a local response to the presence of invading trophoblast.
Journal Article
Cell populations in human early pregnancy decidua: characterization and isolation of large granular lymphocytes by flow cytometry.
TL;DR: Cell populations of human pregnancy decidua, obtained by enzymic digestion from first trimester samples, were analysed by flow cytometry after labelling with monoclonal antibodies, and the NKH1-positive cells were demonstrated to be of similar size to, but slightly higher granularity than, lymphocytes, whereas the CD16- positive cells were larger and more granular.