Journal ArticleDOI
Chondrogenic differentiation of human pluripotent stem cells in chondrocyte co-culture
Chengjuan Qu,Katja A. Puttonen,Heli Lindeberg,Marika Ruponen,Outi Hovatta,Jari Koistinaho,Mikko J. Lammi +6 more
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TLDR
Results show that co-culture of hESCs or hiPSCs with primary chondrocytes could induce specific chondrogenic differentiation, and human CCCs showed osteogenic or adipogenic differentiation capacity.About:
This article is published in The International Journal of Biochemistry & Cell Biology.The article was published on 2013-08-01. It has received 60 citations till now. The article focuses on the topics: Chondrocyte & Embryoid body.read more
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PSEN1 Mutant iPSC-Derived Model Reveals Severe Astrocyte Pathology in Alzheimer's Disease.
Minna Oksanen,Andrew J. Petersen,Nikolay Naumenko,Katja A. Puttonen,Šárka Lehtonen,Max Gubert Olivé,Anastasia Shakirzyanova,Stina Leskelä,Timo Sarajärvi,Matti Viitanen,Matti Viitanen,Juha O. Rinne,Juha O. Rinne,Mikko Hiltunen,Annakaisa Haapasalo,Rashid Giniatullin,Pasi Tavi,Su-Chun Zhang,Katja M. Kanninen,Riikka H. Hämäläinen,Jari Koistinaho +20 more
TL;DR: The generation of functional astrocytes from induced pluripotent stem cells (iPSCs) derived from AD patients with PSEN1 ΔE9 mutation, as well as healthy and gene-corrected isogenic controls are described to highlight the strength of iPSC-derived models for brain diseases.
Journal ArticleDOI
Repair of Damaged Articular Cartilage: Current Approaches and Future Directions.
Ekaterina V Medvedeva,Ekaterina A. Grebenik,Svetlana N Gornostaeva,Vladimir I Telpuhov,Aleksey V. Lychagin,Peter S. Timashev,Andrei S. Chagin +6 more
TL;DR: Current clinical approaches for repairing cartilage, as well as research approaches which are currently developing, and those under translation into clinical practice are discussed, and potential future directions in this area are described, including tissue engineering based on scaffolding and/or stem cells aswell as a combination of gene and cell therapy.
Journal ArticleDOI
Multi-lineage Human iPSC-Derived Platforms for Disease Modeling and Drug Discovery
TL;DR: This work discusses recent advances in generating more complex hiPSC-based systems using three-dimensional organoids, tissue-engineering, microfluidic organ-chips, and humanized animal systems to enable more realistic models of human tissue function.
Journal ArticleDOI
PSEN1ΔE9, APPswe, and APOE4 Confer Disparate Phenotypes in Human iPSC-Derived Microglia
Henna Konttinen,Mauricio Castro Cabral-da-Silva,Sohvi Ohtonen,Sara Wojciechowski,Anastasia Shakirzyanova,Simone Caligola,Rosalba Giugno,Yevheniia Ishchenko,Damián Hernández,Mohammad Feroze Fazaludeen,Shaila Eamen,Mireia Gómez Budia,Ilkka Fagerlund,Flavia Scoyni,Paula Korhonen,Nadine Huber,Annakaisa Haapasalo,Alex W. Hewitt,Alex W. Hewitt,James C. Vickers,Grady C. Smith,Minna Oksanen,Caroline Graff,Katja M. Kanninen,Šárka Lehtonen,Nicholas E. Propson,Michael P. Schwartz,Alice Pébay,Jari Koistinaho,Jari Koistinaho,Lezanne Ooi,Tarja Malm +31 more
TL;DR: It is revealed that the APOE4 genotype has a profound impact on several aspects of microglial functionality, whereas PSEN1ΔE9 and APPswe mutations trigger minor alterations, which indicates that APOE 4 iMGLs are fundamentally unable to mount normal microglia-like cells in AD.
Journal ArticleDOI
Structural Immaturity of Human iPSC-Derived Cardiomyocytes: In Silico Investigation of Effects on Function and Disease Modeling.
Jussi T. Koivumäki,Nikolay Naumenko,Tomi Tuomainen,Jouni Takalo,Minna Oksanen,Katja A. Puttonen,Šárka Lehtonen,Johanna Kuusisto,Markku Laakso,Jari Koistinaho,Pasi Tavi +10 more
TL;DR: Results indicate that hiPSC-CMs translate poorly the disease specific phenotypes of Brugada syndrome, long QT Syndrome and catecholaminergic polymorphic ventricular tachycardia, showing less robustness and greater tendency for arrhythmic events than adult CMs.
References
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
Journal ArticleDOI
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
Journal ArticleDOI
Feeder-free growth of undifferentiated human embryonic stem cells.
Chunhui Xu,Margaret S. Inokuma,Jerrod Denham,Kathaleen Golds,Pratima Kundu,Joseph D. Gold,Melissa K. Carpenter +6 more
TL;DR: A successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least 130 population doublings and are suitable for scaleup production is demonstrated.