Journal ArticleDOI
Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform.
Fengge Song,Yangdao Wei,Peng Wang,Xiaolin Ge,Chaoyang Li,Aimin Wang,Zhiqing Yang,Yi Wan,Yi Wan,Jinghong Li +9 more
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TLDR
In this paper, a universal nucleic acid magneto-DNA nanoparticle system was exploited for the detection of pathogenic bacteria, based on the collateral cleavage activity of CRISPR-Cas14a and tag-specific primer extension.About:
This article is published in Biosensors and Bioelectronics.The article was published on 2021-08-01. It has received 33 citations till now. The article focuses on the topics: Nucleic acid.read more
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CRISPR/Cas-Based In Vitro Diagnostic Platforms for Cancer Biomarker Detection.
TL;DR: In this paper, the authors introduce the working mechanisms of different kinds of CRISPR/Cas systems for biosensing and CRISpl/Cas-mediated detection strategies for different types of cancer biomarkers including nucleic acids, proteins, and extracellular vesicles.
Journal ArticleDOI
CRISPR-Cas-based detection for food safety problems: Current status, challenges, and opportunities.
TL;DR: In this article , a review on CRISPR-Cas-based detection and its current status and huge potential specifically for food safety inspection is presented. But, the current food safety detection methods are still unsatisfactory in some ways such as being timeconsuming, displaying unmet sensitivity and specificity standards, and there is a comparative paucity of multiplexed testing and POCT.
Journal ArticleDOI
Dual-CRISPR/Cas12a-Assisted RT-RAA for Ultrasensitive SARS-CoV-2 Detection on Automated Centrifugal Microfluidics.
TL;DR: A dual-CRISPR/Cas12a-assisted RT-RAA assay and a ″sample-to-answer″ centrifugal microfluidic platform that can automatically detect 1 copy/μL of the SARS-CoV-2 within 30 min, with a significant potential for clinical diagnosis and disease prevention.
Journal ArticleDOI
Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection.
Yangdao Wei,Zhenzhen Tao,L. Wan,Chen Zong,Jiajia Wu,Xiao Tan,Buhua Wang,Zixuan Guo,Ling Zhang,Haoyu Yuan,Peng Wang,Zhiqing Yang,Yi Wan +12 more
TL;DR: Aptamer-based Cas14a1 Biosensor (ACasB) was developed in this paper for the highly specific and sensitive detection of live staphylococcus aureus.
Journal ArticleDOI
Self-powered biosensing system driven by triboelectric nanogenerator for specific detection of Gram-positive bacteria
TL;DR: In this paper , a self-powered biosensing system based on the vertical contact-separation triboelectric nanogenerator (TENG) was developed to specifically detect Gram-positive bacteria in solution.
References
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Journal ArticleDOI
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
Janice S. Chen,Enbo Ma,Lucas B. Harrington,Maria Da Costa,Xinran Tian,Joel M. Palefsky,Jennifer A. Doudna +6 more
TL;DR: It is shown that RNA-guided DNA binding unleashes indiscriminate single-stranded DNA cleavage activity by Cas12a that completely degrades ssDNA molecules, which is also a property of other type V CRISPR-Cas12 enzymes.
Journal ArticleDOI
Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6
Jonathan S. Gootenberg,Omar O. Abudayyeh,Max J. Kellner,Julia Joung,James J. Collins,Feng Zhang +5 more
TL;DR: ShERLOCK as discussed by the authors is a platform that combines isothermal preamplification with Cas13 to detect single molecules of RNA or DNA, which can detect Dengue or Zika virus single-stranded RNA and mutations in patient liquid biopsy samples via lateral flow.
Journal ArticleDOI
RNA editing with CRISPR-Cas13
David Benjamin Turitz Cox,Jonathan S. Gootenberg,Omar O. Abudayyeh,Brian Franklin,Max J. Kellner,Julia Joung,Feng Zhang +6 more
TL;DR: A type VI CRISPR-Cas system containing the programmable single-effector RNA-guided ribonuclease Cas13 is profiled in order to engineer a Cas13 ortholog capable of robust knockdown and REPAIR presents a promising RNA-editing platform with broad applicability for research, therapeutics, and biotechnology.
Journal ArticleDOI
CRISPR-Cas12a-assisted nucleic acid detection.
Shi-Yuan Li,Qiu-Xiang Cheng,Jingman Wang,Xiao-Yan Li,Zilong Zhang,Song Gao,Rui-Bing Cao,Guoping Zhao,Guoping Zhao,Jin Wang +9 more
TL;DR: In a recent study, it was found that Cas 12a, which belongs to the class 2 type V-A CRISPR-Cas system, performed collateral cleavage on non-targeted ssDNAs upon the formation of the Cas12a/crRNA/target DNA ternary complex.
Journal ArticleDOI
Programmed DNA destruction by miniature CRISPR-Cas14 enzymes.
Lucas B. Harrington,David Burstein,Janice S. Chen,David Paez-Espino,Enbo Ma,Isaac P. Witte,Joshua C Cofsky,Nikos C. Kyrpides,Jillian F. Banfield,Jennifer A. Doudna +9 more
TL;DR: Metagenomic data show that multiple CRISPR-Cas14 systems evolved independently and suggest a potential evolutionary origin of single-effector CRISpr-based adaptive immunity, as well as a fast and high-fidelity nucleic acid detection system that enabled detection ofsingle-nucleotide polymorphisms.