Complete Genome Sequence of the Marine Fish Pathogen Vibrio anguillarum Harboring the pJM1 Virulence Plasmid and Genomic Comparison with Other Virulent Strains of V. anguillarum and V. ordalii
Hiroaki Naka,Graciela M. Dias,Cristiane C. Thompson,Christopher Dubay,Fabiano L. Thompson,Jorge H. Crosa +5 more
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TLDR
Mobile genetic elements, some of them possibly originated in the pJM1 plasmid, were very abundant in Vibrio anguillarum 775, resulting in the silencing of specific genes, with only few insertions in the 96F and RV22 chromosomes.Abstract:
We dissected the complete genome sequence of the O1 serotype strain Vibrio anguillarum 775(pJM1) and determined the draft genomic sequences of plasmidless strains of serotype O1 (strain 96F) and O2β (strain RV22) and V. ordalii. All strains harbor two chromosomes, but 775 also harbors the virulence plasmid pJM1, which carries the anguibactin-producing and cognate transport genes, one of the main virulence factors of V. anguillarum. Genomic analysis identified eight genomic islands in chromosome 1 of V. anguillarum 775(pJM1) and two in chromosome 2. Some of them carried potential virulence genes for the biosynthesis of O antigens, hemolysins, and exonucleases as well as others for sugar transport and metabolism. The majority of genes for essential cell functions and pathogenicity are located on chromosome 1. In contrast, chromosome 2 contains a larger fraction (59%) of hypothetical genes than does chromosome 1 (42%). Chromosome 2 also harbors a superintegron, as well as host "addiction" genes that are typically found on plasmids. Unique distinctive properties include homologues of type III secretion system genes in 96F, homologues of V. cholerae zot and ace toxin genes in RV22, and the biofilm formation syp genes in V. ordalii. Mobile genetic elements, some of them possibly originated in the pJM1 plasmid, were very abundant in 775, resulting in the silencing of specific genes, with only few insertions in the 96F and RV22 chromosomes.read more
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Vibrio anguillarum as a fish pathogen: virulence factors, diagnosis and prevention.
TL;DR: This review summarizes the current state of knowledge pertaining to V. anguillarum, focusing on pathogenesis, known virulence factors, diagnosis, prevention and treatment.
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The Genomic Standards Consortium
Dawn Field,Linda A. Amaral-Zettler,Guy Cochrane,James R. Cole,Peter Dawyndt,George M. Garrity,Jack A. Gilbert,Jack A. Gilbert,Frank Oliver Glöckner,Lynette Hirschman,Ilene Karsch-Mizrachi,Hans-Peter Klenk,Rob Knight,Renzo Kottmann,Nikos C. Kyrpides,Folker Meyer,Folker Meyer,Inigo San Gil,Susanna-Assunta Sansone,Lynn M. Schriml,Peter Sterk,Tatiana Tatusova,David W. Ussery,Owen White,John Wooley +24 more
TL;DR: A short history of the GSC is provided, an overview of its range of current activities are provided, and a call for the scientific community to join forces to improve the quality and quantity of contextual information about the authors' public collections of genomes, metagenomes, and marker gene sequences is made.
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