Comprehensive Identification of Cell Cycle–regulated Genes of the Yeast Saccharomyces cerevisiae by Microarray Hybridization
Paul T. Spellman,Gavin Sherlock,Gavin Sherlock,Michael Q. Zhang,Vishwanath R. Iyer,Kirk R. Anders,Michael B. Eisen,Patrick O. Brown,Patrick O. Brown,David Botstein,Bruce Futcher +10 more
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TLDR
A comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle is created, and it is found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins.Abstract:
We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures sync...read more
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Checkpoint proteins control morphogenetic events during DNA replication stress in Saccharomyces cerevisiae
TL;DR: Checkpoint mutants have aberrant cell morphology and cell walls, and show defective bud site selection, and Rad53 shows genetic interactions with septin ring pathway components, and, along with other checkpoint proteins, controls the timely degradation of Swe1 during replication stress, thereby facilitating proper bud growth.
Journal ArticleDOI
Prokaryotic RNA preparation methods useful for high density array analysis: comparison of two approaches
TL;DR: A prokaryotic high density oligonucleotide array using the complete Escherichia coli genome sequence is designed to monitor expression levels of all genes and intergenic regions in the genome to detect the same relative gene expression changes.
Journal ArticleDOI
A Non-Death Role of the Yeast Metacaspase: Yca1p Alters Cell Cycle Dynamics
TL;DR: This work reports that genetic ablation and/or catalytic inactivation of Yca1p leads to a longer G1/S transition accompanied by slower growth in fermentation conditions, and demonstrates multiple non-death roles for yca1 in the cell cycle.
Journal ArticleDOI
Relationship of DFG16 to the Rim101p pH response pathway in Saccharomyces cerevisiae and Candida albicans.
TL;DR: A screen of the Saccharomyces cerevisiae viable deletion mutant library has yielded a new gene required for processed Rim101p accumulation, DFG16, which is homologous to Aspergillus nidulans PalH, a component of the well-characterized PacC processing pathway.
Journal ArticleDOI
The unforeseen challenge: from genotype-to-phenotype in cell populations
TL;DR: A set of concepts underlying cell-state organization-exploration evolving by global, non-specific, dynamics of gene activity-is presented here, which have significant consequences for the understanding of the emergence and stabilization of a cell phenotype in diverse biological contexts.
References
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Cluster analysis and display of genome-wide expression patterns
TL;DR: A system of cluster analysis for genome-wide expression data from DNA microarray hybridization is described that uses standard statistical algorithms to arrange genes according to similarity in pattern of gene expression, finding in the budding yeast Saccharomyces cerevisiae that clustering gene expression data groups together efficiently genes of known similar function.
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TL;DR: DNA microarrays containing virtually every gene of Saccharomyces cerevisiae were used to carry out a comprehensive investigation of the temporal program of gene expression accompanying the metabolic shift from fermentation to respiration, and the expression patterns of many previously uncharacterized genes provided clues to their possible functions.
Book ChapterDOI
Getting started with yeast.
TL;DR: The yeast Saccharomyces cerevisiae is now recognized as a model system representing a simple eukaryote whose genome can be easily manipulated and made particularly accessible to gene cloning and genetic engineering techniques.
Journal ArticleDOI
A Genome-Wide Transcriptional Analysis of the Mitotic Cell Cycle
Raymond J. Cho,Michael J. Campbell,Elizabeth A. Winzeler,Lars M. Steinmetz,Andrew R. Conway,Lisa Wodicka,Tyra G. Wolfsberg,Andrei Gabrielian,David Landsman,David J. Lockhart,Ronald W. Davis +10 more
TL;DR: The genome-wide characterization of mRNA transcript levels during the cell cycle of the budding yeast S. cerevisiae indicates a mechanism for local chromosomal organization in global mRNA regulation and links a range of human genes to cell cycle period-specific biological functions.