Comprehensive Identification of Cell Cycle–regulated Genes of the Yeast Saccharomyces cerevisiae by Microarray Hybridization
Paul T. Spellman,Gavin Sherlock,Gavin Sherlock,Michael Q. Zhang,Vishwanath R. Iyer,Kirk R. Anders,Michael B. Eisen,Patrick O. Brown,Patrick O. Brown,David Botstein,Bruce Futcher +10 more
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TLDR
A comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle is created, and it is found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins.Abstract:
We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures sync...read more
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The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment
TL;DR: A new kinase involved in composing the histone code is revealed and haspin is added to the select group of kinases that integrate regulation of chromosome and spindle function during mitosis and meiosis.
Journal ArticleDOI
A neutral model of transcriptome evolution.
Philipp Khaitovich,Gunter Weiss,Michael Lachmann,Ines Hellmann,Wolfgang Enard,Bjoern Muetzel,Ute Wirkner,Wilhelm Ansorge,Svante Pääbo +8 more
TL;DR: The results suggest that the majority of expression differences observed between species are selectively neutral or nearly neutral and likely to be of little or no functional significance, which should be based on null hypotheses assuming functional neutrality.
Journal ArticleDOI
Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall
TL;DR: Walls biogenesis in vegetative cells is reviewed, covering the structure of wall components and how they are cross-linked; the biosynthesis of N- and O-linked glycans, glycosylphosphatidylinositol membrane anchors, β1,3- and β 1,6-linked glucans, and chitin; the reactions that cross-link wall components; and the possible functions of enzymatic and nonenzymatic cell wall proteins.
Journal ArticleDOI
Histone H2A.Z Regulates Transcription and Is Partially Redundant with Nucleosome Remodeling Complexes
TL;DR: A novel pathway for regulating transcription using variant histones to modulate chromatin structure is described using H2A.Z, which is seen at the level of single genes and acts downstream of promoter nucleosome reorganization.
Journal ArticleDOI
Positive feedback of G1 cyclins ensures coherent cell cycle entry
TL;DR: Positive-feedback-amplified expression of Cln1 and Cln2 simultaneously drives robust budding and rapid, coherent regulon expression, which suggests either conservation of regulatory architecture or convergent evolution.
References
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Cluster analysis and display of genome-wide expression patterns
TL;DR: A system of cluster analysis for genome-wide expression data from DNA microarray hybridization is described that uses standard statistical algorithms to arrange genes according to similarity in pattern of gene expression, finding in the budding yeast Saccharomyces cerevisiae that clustering gene expression data groups together efficiently genes of known similar function.
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TL;DR: DNA microarrays containing virtually every gene of Saccharomyces cerevisiae were used to carry out a comprehensive investigation of the temporal program of gene expression accompanying the metabolic shift from fermentation to respiration, and the expression patterns of many previously uncharacterized genes provided clues to their possible functions.
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Getting started with yeast.
TL;DR: The yeast Saccharomyces cerevisiae is now recognized as a model system representing a simple eukaryote whose genome can be easily manipulated and made particularly accessible to gene cloning and genetic engineering techniques.
Journal ArticleDOI
A Genome-Wide Transcriptional Analysis of the Mitotic Cell Cycle
Raymond J. Cho,Michael J. Campbell,Elizabeth A. Winzeler,Lars M. Steinmetz,Andrew R. Conway,Lisa Wodicka,Tyra G. Wolfsberg,Andrei Gabrielian,David Landsman,David J. Lockhart,Ronald W. Davis +10 more
TL;DR: The genome-wide characterization of mRNA transcript levels during the cell cycle of the budding yeast S. cerevisiae indicates a mechanism for local chromosomal organization in global mRNA regulation and links a range of human genes to cell cycle period-specific biological functions.