Comprehensive Identification of Cell Cycle–regulated Genes of the Yeast Saccharomyces cerevisiae by Microarray Hybridization
Paul T. Spellman,Gavin Sherlock,Gavin Sherlock,Michael Q. Zhang,Vishwanath R. Iyer,Kirk R. Anders,Michael B. Eisen,Patrick O. Brown,Patrick O. Brown,David Botstein,Bruce Futcher +10 more
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TLDR
A comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle is created, and it is found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins.Abstract:
We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures sync...read more
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The fate of the messenger is pre-determined: a new model for regulation of gene expression
TL;DR: An overview of the literature, which provides the evidence of coupling between transcription and decay, review the mechanisms and regulators by which the two processes are coupled, discuss why such coupling is beneficial and present a new model for regulation of gene expression.
Book
Analysis of Microarray Gene Expression Data
TL;DR: This paper presents a meta-modelling framework for supervised learning of DNA, RNA, Proteins, and Gene Expression using microarray data, and some of the methods used in this work came from the literature on self-organizing maps.
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The Transcriptome of Prematurely Aging Yeast Cells Is Similar to That of Telomerase-deficient Cells
TL;DR: The transcriptome of young and old cells isolated by elutriation is analyzed, which allows isolation of biochemical quantities of old cells much further advanced in their life span than old cells prepared by the biotin-streptavidin method, to suggest that old cells are responding to genome instability.
Journal ArticleDOI
Metabolic cycling in single yeast cells from unsynchronized steady-state populations limited on glucose or phosphate
Sanford J. Silverman,Allegra A. Petti,Nikolai Slavov,Lance Parsons,Ryan Briehof,Stephan Y. Thiberge,Daniel Zenklusen,Saumil J. Gandhi,Daniel R. Larson,Robert H. Singer,David Botstein +10 more
TL;DR: The yeast metabolic cycle is an intrinsic property of yeast metabolism and does not depend on either synchronization or external limitation of growth by the carbon source.
Journal ArticleDOI
Emerging technologies in yeast genomics
Anuj Kumar,Michael Snyder +1 more
TL;DR: In this review, a summary of the most promising technologies in yeast genomics is summarized.
References
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Cluster analysis and display of genome-wide expression patterns
TL;DR: A system of cluster analysis for genome-wide expression data from DNA microarray hybridization is described that uses standard statistical algorithms to arrange genes according to similarity in pattern of gene expression, finding in the budding yeast Saccharomyces cerevisiae that clustering gene expression data groups together efficiently genes of known similar function.
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TL;DR: DNA microarrays containing virtually every gene of Saccharomyces cerevisiae were used to carry out a comprehensive investigation of the temporal program of gene expression accompanying the metabolic shift from fermentation to respiration, and the expression patterns of many previously uncharacterized genes provided clues to their possible functions.
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TL;DR: The yeast Saccharomyces cerevisiae is now recognized as a model system representing a simple eukaryote whose genome can be easily manipulated and made particularly accessible to gene cloning and genetic engineering techniques.
Journal ArticleDOI
A Genome-Wide Transcriptional Analysis of the Mitotic Cell Cycle
Raymond J. Cho,Michael J. Campbell,Elizabeth A. Winzeler,Lars M. Steinmetz,Andrew R. Conway,Lisa Wodicka,Tyra G. Wolfsberg,Andrei Gabrielian,David Landsman,David J. Lockhart,Ronald W. Davis +10 more
TL;DR: The genome-wide characterization of mRNA transcript levels during the cell cycle of the budding yeast S. cerevisiae indicates a mechanism for local chromosomal organization in global mRNA regulation and links a range of human genes to cell cycle period-specific biological functions.