Comprehensive Identification of Cell Cycle–regulated Genes of the Yeast Saccharomyces cerevisiae by Microarray Hybridization
Paul T. Spellman,Gavin Sherlock,Gavin Sherlock,Michael Q. Zhang,Vishwanath R. Iyer,Kirk R. Anders,Michael B. Eisen,Patrick O. Brown,Patrick O. Brown,David Botstein,Bruce Futcher +10 more
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TLDR
A comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle is created, and it is found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins.Abstract:
We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures sync...read more
Citations
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Proceedings ArticleDOI
Cluster stability and the use of noise in interpretation of clustering
TL;DR: A clustering and ordination algorithm suitable for mining extremely large databases, including those produced by microarray expression studies, is described and analyzed for stability.
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ACE2, CBK1, and BUD4 in budding and cell separation.
TL;DR: It is shown that an ACE2 multicopy plasmid suppresses the latter three defects of RAM network mutations, demonstrating that Ace2 is downstream of the RAM network and suggesting that these phenotypes are caused by reduced expression of Ace2 target genes.
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DNA replication stress differentially regulates G1/S genes via Rad53-dependent inactivation of Nrm1
Anna Travesa,Dwight Kuo,Robertus A.M. de Bruin,Tatyana I. Kalashnikova,Marisela Guaderrama,Kevin K. Thai,Aaron Aslanian,Marcus B. Smolka,John R. Yates,Trey Ideker,Curt Wittenberg +10 more
TL;DR: It is concluded that MBF‐regulated genes are distinguished from SBF‐ regulated genes by their sensitivity to activation by the S‐phase checkpoint, thereby, providing an effective mechanism for enhancing DNA replication and repair and promoting genome stability.
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Mismatch repair proteins and mitotic genome stability.
TL;DR: An overview of the anti-mutator and anti-recombination functions of MMR proteins in the yeast Saccharomyces cerevisiae is provided.
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Heritable capture of heterochromatin dynamics in Saccharomyces cerevisiae
Anne E. Dodson,Jasper Rine +1 more
TL;DR: A genetic strategy to capture transient losses of gene silencing in Saccharomyces as permanent, heritable changes in genotype and phenotype and uncovered previously unappreciated roles for Sir1, a silencing establishment factor, in the maintenance and/or inheritance of silencing.
References
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TL;DR: A system of cluster analysis for genome-wide expression data from DNA microarray hybridization is described that uses standard statistical algorithms to arrange genes according to similarity in pattern of gene expression, finding in the budding yeast Saccharomyces cerevisiae that clustering gene expression data groups together efficiently genes of known similar function.
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TL;DR: DNA microarrays containing virtually every gene of Saccharomyces cerevisiae were used to carry out a comprehensive investigation of the temporal program of gene expression accompanying the metabolic shift from fermentation to respiration, and the expression patterns of many previously uncharacterized genes provided clues to their possible functions.
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TL;DR: The yeast Saccharomyces cerevisiae is now recognized as a model system representing a simple eukaryote whose genome can be easily manipulated and made particularly accessible to gene cloning and genetic engineering techniques.
Journal ArticleDOI
A Genome-Wide Transcriptional Analysis of the Mitotic Cell Cycle
Raymond J. Cho,Michael J. Campbell,Elizabeth A. Winzeler,Lars M. Steinmetz,Andrew R. Conway,Lisa Wodicka,Tyra G. Wolfsberg,Andrei Gabrielian,David Landsman,David J. Lockhart,Ronald W. Davis +10 more
TL;DR: The genome-wide characterization of mRNA transcript levels during the cell cycle of the budding yeast S. cerevisiae indicates a mechanism for local chromosomal organization in global mRNA regulation and links a range of human genes to cell cycle period-specific biological functions.