Development of Reverse Transcription Loop-Mediated Isothermal Amplification Assays Targeting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2).
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TLDR
R reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays are developed and evaluated to detect genomic RNA of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative virus of COVID-19.About:
This article is published in The Journal of Molecular Diagnostics.The article was published on 2020-06-01 and is currently open access. It has received 328 citations till now. The article focuses on the topics: Reverse Transcription Loop-mediated Isothermal Amplification & Loop-mediated isothermal amplification.read more
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Trends in biosensing platforms for SARS-CoV-2 detection: A critical appraisal against standard detection tools.
TL;DR: In this ongoing theme of COVID-19 pandemic, highly sensitive analytical testing platforms are extremely necessary to detect SARS-CoV-2 RNA and antiviral antibodies and the vitality of biosensing technologies emphasizing on optical and electrochemical biosensors towards the detection of Sars-Cov-2 has been discussed here.
Journal ArticleDOI
Colorimetric RT-LAMP SARS-CoV-2 diagnostic sensitivity relies on color interpretation and viral load.
Mateus Nóbrega Aoki,Bruna de Oliveira Coelho,Luiz Gustavo Bentim Góes,Paola Minoprio,Edison Luiz Durigon,Luis Gustavo Morello,Fabricio K Marchini,Irina Natassja Riediger,Maria do Carmo Debur,Helder I. Nakaya,Lucas Blanes +10 more
TL;DR: In this paper, the authors show that the RT-LAMP reaction has a sensitivity of only 200 RNA virus copies, with a color change from pink to yellow occurring in 100% of the 62 clinical samples tested positive by RT-qPCR.
Journal ArticleDOI
Laboratory diagnosis of SARS-CoV-2: available approaches and limitations
TL;DR: A handful of serological tests have granted authorization to be used under emergency situation by FDA in diagnosis of SARS-CoV-2 and are expected to be helpful in pandemic management since no particular skills are required from the operator.
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Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks.
TL;DR: A fast and affordable method to purify nucleic acids from animal, plant, viral and microbial samples using a cellulose-based dipstick, ideally suited for molecular applications, both within and outside the laboratory.
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Point of Care Diagnostics in the Age of COVID-19
Meysam Rezaei,Sajad Razavi Bazaz,Sareh Zhand,Nima Sayyadi,Dayong Jin,Martin P. Stewart,Majid Ebrahimi Warkiani +6 more
TL;DR: Point-of-care (POC) tests have the potential to vastly improve healthcare in several ways, ranging from enabling earlier detection and easier monitoring of disease to reaching remote populations as mentioned in this paper.
References
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MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets
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A pneumonia outbreak associated with a new coronavirus of probable bat origin
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TL;DR: Phylogenetic and metagenomic analyses of the complete viral genome of a new coronavirus from the family Coronaviridae reveal that the virus is closely related to a group of SARS-like coronaviruses found in bats in China.
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