Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development.
Charles E. Murry,Gordon Keller +1 more
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TLDR
The potential to generate virtually any differentiated cell type from embryonic stem cells (ESCs) offers the possibility to establish new models of mammalian development and to create new sources of cells for regenerative medicine, but it is essential to be able to control ESC differentiation and to direct the development of these cells along specific pathways.About:
This article is published in Cell.The article was published on 2008-02-22 and is currently open access. It has received 1695 citations till now. The article focuses on the topics: Embryoid body & Adult stem cell.read more
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Growth Factors, Matrices, and Forces Combine and Control Stem Cells
TL;DR: Multifaceted technologies are increasingly required to produce and interrogate cells ex vivo, to build predictive models, and, ultimately, to enhance stem cell integration in vivo for therapeutic benefit.
Journal ArticleDOI
Disease-Specific Induced Pluripotent Stem Cells
In-Hyun Park,Natasha Arora,Hongguang Huo,Nimet Maherali,Tim Ahfeldt,Tim Ahfeldt,Akiko Shimamura,M. William Lensch,M. William Lensch,Chad A. Cowan,Konrad Hochedlinger,George Q. Daley +11 more
TL;DR: The generation of induced pluripotent stem cells from patients with a variety of genetic diseases with either Mendelian or complex inheritance are described, offering an unprecedented opportunity to recapitulate both normal and pathologic human tissue formation in vitro, thereby enabling disease investigation and drug development.
Journal ArticleDOI
Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling
Xiaojun Lian,Cheston Hsiao,Gisela F. Wilson,Kexian Zhu,Laurie B. Hazeltine,Samira M. Azarin,Kunil K. Raval,Kunil K. Raval,Jianhua Zhang,Jianhua Zhang,Timothy J. Kamp,Timothy J. Kamp,Sean P. Palecek +12 more
TL;DR: It is shown that temporal modulation of Wnt signaling is both essential and sufficient for efficient cardiac induction in hPSCs under defined, growth factor-free conditions.
Journal ArticleDOI
In vivo reprogramming of murine cardiac fibroblasts into induced cardiomyocytes
Li Qian,Yu Huang,C. Ian Spencer,Amy Foley,Vasanth Vedantham,Lei Liu,Simon J. Conway,Ji-Dong Fu,Deepak Srivastava +8 more
TL;DR: In this article, the authors used genetic lineage tracing to show that resident nonmyocytes in the murine heart can be reprogrammed into cardiomyocyte-like cells in vivo by local delivery of GMT after coronary ligation.
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Stage-specific optimization of activin/nodal and BMP signaling promotes cardiac differentiation of mouse and human pluripotent stem cell lines.
Steven J. Kattman,Alec D. Witty,Alec D. Witty,Mark Gagliardi,Nicole Dubois,Maryam Niapour,Akitsu Hotta,James Ellis,Gordon Keller +8 more
TL;DR: It is found that individual mouse and human pluripotent stem cell lines require optimization of these signaling pathways for efficient cardiac differentiation, illustrating a principle that may well apply in other contexts.
References
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
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Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
Journal ArticleDOI
Induction of Pluripotent Stem Cells From Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: This work generated induced pluripotent stem cells capable of germline transmission from murine somatic cells by transd, and demonstrated the ability of these cells to reprogram into patient-specific and disease-specific stem cells.
Supporting Online Material for Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron M. Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: Yu et al. as discussed by the authors proposed online material for induced pluripotent stem cell lines derived from human Somatic Cells, which can be used for transplanting human stem cells to humans.