Efficient population modification gene-drive rescue system in the malaria mosquito Anopheles stephensi.
Adriana Adolfi,Adriana Adolfi,Valentino M. Gantz,Nijole Jasinskiene,H. K. Lee,Kristy S. Hwang,Gerard Terradas,Emily A. Bulger,Arunachalam Ramaiah,Jared B. Bennett,J. J. Emerson,John M. Marshall,Ethan Bier,Anthony A. James +13 more
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TLDR
A recoded gene-drive rescue system for population modification of the malaria vector, Anopheles stephensi, that relieves the load in females caused by integration of the drive into the kynurenine hydroxylase gene by rescuing its function is developed.Abstract:
Cas9/gRNA-mediated gene-drive systems have advanced development of genetic technologies for controlling vector-borne pathogen transmission. These technologies include population suppression approaches, genetic analogs of insecticidal techniques that reduce the number of insect vectors, and population modification (replacement/alteration) approaches, which interfere with competence to transmit pathogens. Here, we develop a recoded gene-drive rescue system for population modification of the malaria vector, Anopheles stephensi, that relieves the load in females caused by integration of the drive into the kynurenine hydroxylase gene by rescuing its function. Non-functional resistant alleles are eliminated via a dominantly-acting maternal effect combined with slower-acting standard negative selection, and rare functional resistant alleles do not prevent drive invasion. Small cage trials show that single releases of gene-drive males robustly result in efficient population modification with ≥95% of mosquitoes carrying the drive within 5-11 generations over a range of initial release ratios.read more
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Gene-drive suppression of mosquito populations in large cages as a bridge between lab and field.
Andrew Hammond,Andrew Hammond,Paola Pollegioni,Tania Persampieri,Ace North,Roxana Minuz,Alessandro Trusso,Alessandro Bucci,Kyros Kyrou,Ioanna Morianou,Alekos Simoni,Tony Nolan,Tony Nolan,Ruth Müller,Ruth Müller,Andrea Crisanti +15 more
TL;DR: In this article, the authors report the suppressive activity of the gene-drive in age-structured An. gambiae populations in large indoor cages that permit complex feeding and reproductive behaviours.
Journal ArticleDOI
A confinable home and rescue gene drive for population modification
TL;DR: In this paper, a confinable population modification home-and-rescue (HomeR) drive was proposed for population modification in Drosophila targeting an essential gene.
Journal ArticleDOI
Adequacy and sufficiency evaluation of existing EFSA guidelines for the molecular characterisation, environmental risk assessment and post-market environmental monitoring of genetically modified insects containing engineered gene drives.
Hanspeter Naegeli,Jean-Louis Bresson,Tamas Dalmay,Ian Crawford Dewhurst,Michelle M. Epstein,Philippe Guerche,Jan Hejatko,Francisco Javier Moreno,Ewen Mullins,Fabien Nogué,Nils Rostoks,Jose Juan Sánchez Serrano,Giovanni Savoini,Eve Veromann,Fabio Veronesi,Michael B. Bonsall,John D. Mumford,Ernst A. Wimmer,Yann Devos,Konstantinos Paraskevopoulos,Leslie George Firbank +20 more
TL;DR: In this Scientific Opinion, the Panel on Genetically Modified Organisms (GMO) concludes that EFSA's guidelines are adequate, but insufficient for the molecular characterisation, environmental risk assessment and post‐market environmental monitoring of GDMIs.
Journal ArticleDOI
Design and analysis of CRISPR-based underdominance toxin-antidote gene drives.
TL;DR: The results suggest that CRISPR TA underdominance drive systems could allow for the design of flexible and potentially confinable gene drive strategies, and provide a wide range of invasion threshold frequencies and options for both population modification and suppression.
Journal ArticleDOI
Symbionts and gene drive: two strategies to combat vector-borne disease.
TL;DR: In this paper , Bhatt et al. reviewed the latest developments in both symbionts and gene drive-based methods, as well as distinctions and obstacles relating to these promising technologies.
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