Journal ArticleDOI
Evidence for the T3-associated 90K heterodimer as the T-cell antigen receptor
Stefan Meuer,Oreste Acuto,Rebecca E. Hussey,James C. Hodgdon,K A Fitzgerald,Stuart F. Schlossman,Ellis L. Reinherz +6 more
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TLDR
It is suggested that each T lymphocyte, regardless of subset derivation or specificity, uses an analogous Ti heterodimer for antigen specific function.Abstract:
Several surface molecules appear to be involved in antigen recognition by human T lymphocytes including the monomorphic 20/25K T3 structure present on all mature T lymphocytes and the subset-specific associative recognition elements, T4 and T8 (refs 1–8). More recently, Ti1, a clonally unique antigen recognition structure comprised of a 49,000 molecular weight (49K) α-chain and a 43K β-chain, linked to T3 was identified on a major histocompatibility complex (MHC) class I specific T8+ T-cell clone, CT8III (ref. 9). To determine whether analogous receptor molecules could be found on other T-cell clones of differing specificity, we produced monoclonal antibodies against a clonal structure (Ti2) on an MHC class II specific T4+ lymphocyte, CT4II, derived from the same donor as CT8III. The Ti2 structure on CT4II is shown here to be a disulphide-linked heterodimer like Ti1 on CT8III and is composed of summits of similar molecular weight. Monoclonal antibodies against Ti2 or Ti1 block antigen specific functions of the respective clone without showing any cross-reactivity. These findings suggest that each T lymphocyte, regardless of subset derivation or specificity, uses an analogous Ti heterodimer for antigen specific function. The latter is linked to T3 and expressed on the cell surface at an identical density (30,000–40,000 sites per cell).read more
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Journal ArticleDOI
Interleukin-2: inception, impact, and implications.
TL;DR: Because T cell clonal proliferation after antigen challenge is obligatory for immune responsiveness and immune memory, the IL-2-T cell system has opened the way to a molecular understanding of phenomena that are fundamental to biology, immunology, and medicine.
Journal ArticleDOI
Isolation of cDNA clones encoding T cell-specific membrane-associated proteins.
Stephen M. Hedrick,Stephen M. Hedrick,David I. Cohen,David I. Cohen,Ellen A. Nielsen,Mark M. Davis,Mark M. Davis +6 more
TL;DR: Of 10 distinct cloned DNA copies of mRNAs expressed in T lymphocytes but not in B lymphocytes and associated with membrane-bound polysomes, one hybridizes to a region of the genome that has rearranged in a T- cell lymphoma and several T-cell hybridomas, suggesting that it encodes one chain of the elusive antigen receptor on the surface of T lymphocyte.
Journal ArticleDOI
An alternative pathway of T-cell activation: A functional role for the 50 kd T11 sheep erythrocyte receptor protein
Stefan Meuer,Rebecca E. Hussey,Marina Fabbi,David A. Fox,Oreste Acuto,K A Fitzgerald,James C. Hodgdon,Jeffrey P. Protentis,Stuart F. Schlossman,Ellis L. Reinherz +9 more
TL;DR: Given that T11 is the earliest T-lineage surface glycoprotein to appear in thymic ontogeny and is thus expressed before T3-Ti, the former may be involved in clonal expansion and/or differentiation during early development.
Journal ArticleDOI
T cell costimulatory receptor CD28 is a primary target for PD-1-mediated inhibition.
Enfu Hui,Jeanne Cheung,Jing Zhu,Xiaolei Su,Marcus J. Taylor,Heidi J.A. Wallweber,Dibyendu Kumar Sasmal,Jun Huang,Jeong M. Kim,Ira Mellman,Ronald D. Vale +10 more
TL;DR: It is shown that the co-receptor CD28 is strongly preferred over the TCR as a target for dephosphorylation by PD-1–recruited Shp2 phosphatase, suggesting that costimulatory pathways play key roles in regulating effector T cell function and responses to anti-PD-L1/PD-1 therapy.
Journal ArticleDOI
Limited heterogeneity of T cell receptors from lymphocytes mediating autoimmune encephalomyelitis allows specific immune intervention
Hans Acha-Orbea,Dennis J. Mitchell,Luika Timmermann,David C. Wraith,Gilbert S. Tausch,Matthew K. Waldor,Scott S. Zamvil,Hugh O. McDevitt,Lawrence Steinman +8 more
TL;DR: Prevention and reversal of autoimmune disease with V beta 8-specific monoclonal antibodies was achieved in EAE because of a striking similarity in fine specificity of T cell receptors.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Continuous cultures of fused cells secreting antibody of predefined specificity
G Köhler,C. Milstein +1 more
TL;DR: The derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies is described here, made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor.
Journal ArticleDOI
Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose.
TL;DR: A simple and rapid method for isolating pure mouse IgG1, IgG 2a and IgG2b immunoglobulins in nearly 100% yield is described and all IgG was retained at pH 8.0 and this could not be eluted by washing.
Journal ArticleDOI
The differentiation and function of human T lymphocytes
TL;DR: The precise dissection of cellular mechanisms and interactions involved in the generation of human T cell responses has been facilitated in recent years by advances in four areas: first, the development of in this paper.