Journal ArticleDOI
Evidence for transposition of dispersed repetitive DNA families in yeast
TLDR
In this paper, Dispersed repetitive DNA sequences from yeast (Saccharomyces cerevisiae) nuclear DNA have been isolated as molecular hybrids in lambdagt and showed marked alterations in the size of the restriction fragments containing these repetitive DNAs.About:
This article is published in Cell.The article was published on 1979-04-01. It has received 446 citations till now. The article focuses on the topics: Repeated sequence & Restriction fragment.read more
Citations
More filters
Journal ArticleDOI
A cDNA clone from Zea mays endosperm sucrose synthetase mRNA.
TL;DR: A cDNA clone for maize endosperm sucrose synthetase of 62o nucleotide pairs length was obtained by cloning double stranded DNA obtained from the total maize endOSperm poly(A) RNA in pBR322, and identifying the appropriate clone by hybrid-promoter translation.
Journal ArticleDOI
Genetic variation among European strains of Saccharomyces paradoxus: results from DNA fingerprinting.
TL;DR: Microsatellite fingerprinting and RAPD analysis was used to characterize 28 wild European strains of Saccharomyces paradoxus and revealed extensive genetic variation, allowing discrimination to the strain level.
Journal ArticleDOI
Effects of point mutations in a hinge region on the stability, folding, and enzymatic activity of Escherichia coli dihydrofolate reductase.
TL;DR: Results suggest that side-chain interactions in the interior of the turn influence the folding and stability of dihydrofolate reductase, and possibly also between various forms of the apoenzyme.
Journal ArticleDOI
Frequent site-specific deletion of coliphage lambda murine sarcoma virus recombinants and its use in the identification of a retrovirus integration site.
TL;DR: An analysis of the heteroduplexes between the integration site and the lambdaHT1r+ deletion indicated that no major rearrangement of host sequences occurred upon integration of the Moloney murine sarcoma provirus.
Journal ArticleDOI
Centromeric DNA from Saccharomyces uvarum is functional in Saccharomyces cerevisiae
TL;DR: DNA hybridization to separated chromosomal DNAs revealed that the two newly determined S. cerevisiae centromeric DNA sequences belong to chromosomes V and XIII, respectively.
References
More filters
Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
TL;DR: A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
Book ChapterDOI
[31] Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids
Journal ArticleDOI
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
TL;DR: Electrophoresis in alkaline gels can provide accurate molecular weights for linear, single-Stranded DNAs, and should be useful in analyzing DNA for single-strand breaks, depurinations or topological differences such as ring forms.
Journal ArticleDOI
Chromosome organization and genic expression.
TL;DR: Presented at the Cold Spring Harbor Symposium in 1951, this paper focused on regulation and development rather than transposition, and strongly reflected McClintock's newfound interest in theory.