Journal ArticleDOI
Evidence for transposition of dispersed repetitive DNA families in yeast
TLDR
In this paper, Dispersed repetitive DNA sequences from yeast (Saccharomyces cerevisiae) nuclear DNA have been isolated as molecular hybrids in lambdagt and showed marked alterations in the size of the restriction fragments containing these repetitive DNAs.About:
This article is published in Cell.The article was published on 1979-04-01. It has received 446 citations till now. The article focuses on the topics: Repeated sequence & Restriction fragment.read more
Citations
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Journal ArticleDOI
The DNA intermediate in yeast Ty1 element transposition copurifies with virus-like particles: Cell-free Ty1 transposition
Daniel Eichinger,Jef D. Boeke +1 more
TL;DR: A cell-free system for transposition of Ty1 DNA molecules into a bacteriophage lambda target is developed and mutations in the integrase coding region abolish transposition both in vivo and in vitro.
Journal ArticleDOI
Genetic homology between Saccharomyces cerevisiae and its sibling species S. paradoxus and S. bayanus: electrophoretic karyotypes.
TL;DR: Chromosomal DNAs of many monosporic strains of the biological species Saccharomyces cerevisiae, S. paradoxus and S. bayanus were analysed using contour‐clamped homogeneous electric field electrophgoresis and a comparative study of Ty1, Ty2 and telomere‐associated Y' sequences having multiple chromosomal location was done.
Journal ArticleDOI
Survey of molecular methods for the typing of wine yeast strains
TL;DR: A survey of the genetic polymorphisms produced by distinct methods was performed in 23 commercial winery yeast strains, finding that any of the methods can be applied at the industrial scale.
Journal ArticleDOI
The SPT3 gene is required for normal transcription of Ty elements in S. cerevisiae
TL;DR: The requirement of SPT3 for normal transcription from delta can explain the ability of spt3 mutations to suppress the mutations caused by Ty and delta insertions and lead to defects in diploid formation and sporulation, suggesting thatSPT3 is important for the expression of genes in addition to Ty elements.
Journal ArticleDOI
Rearranged mitochondrial genes in the yeast nuclear genome.
Frances Farrelly,Ronald A. Butow +1 more
TL;DR: A contiguous DNA sequence in the yeast nuclear genome with extensive homology to non-contiguous yeast mitochondrial DNA sequences is found and certain features of the content and organization suggest that it may have originated from petite mitochondrial DNA which integrated into the nuclear genome.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
TL;DR: A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
Book ChapterDOI
[31] Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids
Journal ArticleDOI
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
TL;DR: Electrophoresis in alkaline gels can provide accurate molecular weights for linear, single-Stranded DNAs, and should be useful in analyzing DNA for single-strand breaks, depurinations or topological differences such as ring forms.
Journal ArticleDOI
Chromosome organization and genic expression.
TL;DR: Presented at the Cold Spring Harbor Symposium in 1951, this paper focused on regulation and development rather than transposition, and strongly reflected McClintock's newfound interest in theory.