Journal ArticleDOI
Evidence for transposition of dispersed repetitive DNA families in yeast
TLDR
In this paper, Dispersed repetitive DNA sequences from yeast (Saccharomyces cerevisiae) nuclear DNA have been isolated as molecular hybrids in lambdagt and showed marked alterations in the size of the restriction fragments containing these repetitive DNAs.About:
This article is published in Cell.The article was published on 1979-04-01. It has received 446 citations till now. The article focuses on the topics: Repeated sequence & Restriction fragment.read more
Citations
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Journal ArticleDOI
Evolutionary Genomics of Transposable Elements in Saccharomyces cerevisiae
TL;DR: The results demonstrate that the genome of a single individual contains important information about the state of TE population dynamics within a species and suggest that horizontal transfer may play an important role in shaping the genomic diversity of transposable elements in unicellular eukaryotes.
Book ChapterDOI
The Differentiation of Germ and Somatic Cell Lines in Nematodes
TL;DR: This review will focus on a discussion of germ line versus soma differentiation, and it should be emphasized that the underlying mechanism(s) involved in the differentiation of the divergent somatic cell lines may in principle be similar.
Journal ArticleDOI
Genetic regulation of telomere-telomere fusions in the yeast Saccharomyces cerevisae
TL;DR: Using a PCR assay, it is demonstrated that mec1 tel1 strains also have telomere–telomere fusions (T-TFs), which argue that many of these deletions reflect a cycle of T-TF formation (resulting in dicentric chromosomes), followed by chromosome breakage.
Journal ArticleDOI
Spontaneous unstable unc-22 IV mutations in C. elegans var. Bergerac.
TL;DR: It is suggested that this mutator system is polygenic and dispersed over the nematode genome and could represent activity of the transposable element Tc1.
Journal ArticleDOI
Concerted deletions and inversions are caused by mitotic recombination between delta sequences in Saccharomyces cerevisiae.
R Rothstein,C Helms,N Rosenberg +2 more
TL;DR: Two gene conversion mechanisms by which deletions between repeated sequences in Saccharomyces cerevisiae containing mutations in the recombination and repair gene RAD52 could have been generated are presented.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
TL;DR: A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
Book ChapterDOI
[31] Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids
Journal ArticleDOI
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
TL;DR: Electrophoresis in alkaline gels can provide accurate molecular weights for linear, single-Stranded DNAs, and should be useful in analyzing DNA for single-strand breaks, depurinations or topological differences such as ring forms.
Journal ArticleDOI
Chromosome organization and genic expression.
TL;DR: Presented at the Cold Spring Harbor Symposium in 1951, this paper focused on regulation and development rather than transposition, and strongly reflected McClintock's newfound interest in theory.