Journal ArticleDOI
Evidence for transposition of dispersed repetitive DNA families in yeast
TLDR
In this paper, Dispersed repetitive DNA sequences from yeast (Saccharomyces cerevisiae) nuclear DNA have been isolated as molecular hybrids in lambdagt and showed marked alterations in the size of the restriction fragments containing these repetitive DNAs.About:
This article is published in Cell.The article was published on 1979-04-01. It has received 446 citations till now. The article focuses on the topics: Repeated sequence & Restriction fragment.read more
Citations
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Overexpression and secretion of cellulolytic enzymes by δ-sequence-mediated multicopy integration of heterologous DNA sequences into the chromosomes of Saccharomyces cerevisiae
Daisuke Mochizuki,Kohji Miyahara,Dai Hirata,Hiroaki Matsuzaki,Takushi Hatano,Sakuzo Fukui,Tokichi Miyakawa +6 more
TL;DR: It is suggested that the δ-sequence-mediated integration system is a very useful means for the genetic engineering of yeast, especially when overproduction and secretion of multiple heterologous enzymes are desired.
Journal ArticleDOI
Control of yeast gene expression by transposable elements: maximum expression requires a functional Ty activator sequence and a defective Ty promoter.
L R Coney,G S Roeder +1 more
TL;DR: Results of these experiments indicated that a sequence element is present in the Ty epsilon region that is necessary for HIS4 expression but which has only a modest effect on Ty transcription.
Journal ArticleDOI
A simple method for the rapid purification of copia virus-like particles from Drosophila Schneider 2 cells
TL;DR: The results show that copia particles are mainly located in the nucleus of S2 cells, and this new protocol may find broad applications for the purification of various other VLPs, and thus, may be of great value to other investigators with similar interests.
Journal ArticleDOI
Constitutive expression of the maltose fermentative enzymes in Saccharomyces carlsbergensis is dependent upon the mutational activation of a nonessential homolog of MAL63
TL;DR: The results revealed that the MAL64-C alleles present in constitutive strains encode a trans-acting positive function required for constitutive expression of the Mal61 and MAL62 gene products, suggesting that they control MAL61 andMAL62 transcript accumulation by similar mechanisms.
Journal ArticleDOI
Improved Stress Tolerance of Saccharomyces cerevisiae by CRISPR-Cas-Mediated Genome Evolution
TL;DR: This study developed a novel genome shuffling method for S. cerevisiae using CRISPR-Cas and created a thermotolerant mutant strain T8-292, which can grow well at 39 °C and showed higher cell viability in low pH and high ethanol concentration.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
TL;DR: A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
Book ChapterDOI
[31] Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids
Journal ArticleDOI
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
TL;DR: Electrophoresis in alkaline gels can provide accurate molecular weights for linear, single-Stranded DNAs, and should be useful in analyzing DNA for single-strand breaks, depurinations or topological differences such as ring forms.
Journal ArticleDOI
Chromosome organization and genic expression.
TL;DR: Presented at the Cold Spring Harbor Symposium in 1951, this paper focused on regulation and development rather than transposition, and strongly reflected McClintock's newfound interest in theory.