Journal ArticleDOI
Evidence for transposition of dispersed repetitive DNA families in yeast
TLDR
In this paper, Dispersed repetitive DNA sequences from yeast (Saccharomyces cerevisiae) nuclear DNA have been isolated as molecular hybrids in lambdagt and showed marked alterations in the size of the restriction fragments containing these repetitive DNAs.About:
This article is published in Cell.The article was published on 1979-04-01. It has received 446 citations till now. The article focuses on the topics: Repeated sequence & Restriction fragment.read more
Citations
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Journal ArticleDOI
Ty1 Proteolytic Cleavage Sites Are Required for Transposition: All Sites Are Not Created Equal
TL;DR: To determine the role of individual protease cleavage sites in Ty1, a retrotransposon from Saccharomyces cerevisiae, the Cleavage sites were systematically mutagenized and it was found that the digestion of the Gag/PR site and release of the protease N terminus is a prerequisite for processing at the remaining sites.
Journal ArticleDOI
Genes in Neurospora that suppress recombination when they are heterozygous.
TL;DR: It is proposed that ss (synaptic sequence) genes modulate recombination by determining the pairing closeness of DNA duplexes in the vicinity of the nit-2 locus.
Journal ArticleDOI
The 1723 element: A long, homogeneous, highly repeated DNA unit interspersed in the genome of Xenopus laevis
Brian K. Kay,Igor B. Dawid +1 more
TL;DR: A highly repeated DNA element in the Xenopus laevis genome, named the 1723 element, which was first identified among sequences that are transcribed during embryonic development is described and suggests that it may be transposable.
Journal ArticleDOI
The molecular characterization of three transcriptional mutations in the yeast iso-2-cytochrome c gene.
TL;DR: Three mutations, each of which causes overproduction of iso-2-cytochrome c, were characterized biochemically and found to be caused by the integration of a transposable element, Tyl, 269 base pairs 5' to the coding sequences.
Journal ArticleDOI
Breeding of a new type of baker's yeast by δ-integration for overproduction of glucoamylase using a homothallic yeast
TL;DR: A new type of integrative plasmid was constructed by the δ-integration system using a δ sequence of the yeast retrotransposon Ty1 to obtain transformants with higher glucoamylase production ability and could grow in synthetic medium containing starch as a carbon source.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
TL;DR: A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
Book ChapterDOI
[31] Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids
Journal ArticleDOI
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
TL;DR: Electrophoresis in alkaline gels can provide accurate molecular weights for linear, single-Stranded DNAs, and should be useful in analyzing DNA for single-strand breaks, depurinations or topological differences such as ring forms.
Journal ArticleDOI
Chromosome organization and genic expression.
TL;DR: Presented at the Cold Spring Harbor Symposium in 1951, this paper focused on regulation and development rather than transposition, and strongly reflected McClintock's newfound interest in theory.